Scn3b在遗传性糖尿病长爪沙鼠多种组织中的表达情况  

Expression Pattern of Scn3b in Multiple Tissues from Hereditary Diabetic Mongolian Gerbils

作　　者：赵培琨 龚菁菁[1] 杜小燕[1] 李长龙[1] 霍学云[1] 路静[1] 吕建祎[1] 刘欣[1] 陈振文[1] 郭萌[1] ZHAO Peikun;GONG Jingjing;DU Xiaoyan;LI Changlong;HUO Xueyun;LU Jianyi;LIU Xin;CHEN Zhenwen;GUO Meng(School of Basic Medical Science, Capital University of Medical Sciences, Beijing 100069, China)

机构地区：[1]首都医科大学基础医学院,北京100069

出　　处：《实验动物科学》2018年第2期11-15,共5页Laboratory Animal Science

基　　金：国家自然科学基金(No.31272393;31402027);国家科技支撑计划(No.2015BAI09B01);北京自然基金(No.7141002)

摘　　要：目的比较正常血糖长爪沙鼠和自发遗传性糖尿病长爪沙鼠肝脏、肾脏、骨骼肌和脑组织中钠通道β亚基(sodium channel beta subunit 3 gene,Scn3b)的表达差异。方法选取正常血糖长爪沙鼠和自发遗传性糖尿病长爪沙鼠各6只,分别采集动物的肝脏、肾脏、骨骼肌和脑组织,利用Real-time PCR和Western blotting检测Scn3b在各组织中的mRNA和蛋白表达水平。结果与正常血糖动物相比,在糖尿病长爪沙鼠肝脏和脑组织中,Scn3b mRNA和蛋白水平表达均下降,其中在肝脏具显著性差异。结论 Scn3b在糖尿病长爪沙鼠肝脏和脑组织中表达减少,提示在肝脏和脑组织中Scn3b的异常表达可能参与长爪沙鼠糖尿病的发生。Objective To investigate the mRNA and protein expression level of sodium channel beta subunit 3 gene（Scn3b） in 4 tissues of spontaneous hereditary diabetic and non-diabetic gerbils. Method We selected 6 diabetic and 6 non-diabetic control gerbils,and collected liver,kidney,skeletal muscle and brain from each animal,respectively. The mRNA and protein expression of Scn3 b was evaluated by Real-time PCR and Western blotting in diabetic and non-diabetic control groups. Result Compared with control group,mRNA and protein expression of Scn3b reduced in liver and brain from diabetic gerbils,and the difference in liver between the two groups was statistical significant. Conclusion Scn3b expression decreased in liver and brain in diabetic gerbils,suggesting that Scn3 b in liver and brain may be involved in development of gerbil diabetes.

关 键 词：长爪沙鼠 2型糖尿病 Scn3b REAL-TIME PCR WESTERN BLOTTING 

分 类 号：R-33[医药卫生]