经胃左动脉注入VX2瘤粒建立兔胃癌模型  

The establishment of rabbit model of gastric cancer by injecting VX2 tumor particles through left gastric artery

作　　者：冯国栋 余辉[1] 冯建防[2] 周斌[3] 席玮[1] 武贝[1] 张静渊[4] 陈环球[3] 严枫[5] 陈骏[1] FENG Guodong;YU Hui;FENG Jianfang;ZHOU Bin;XI Wei;WU Bei;ZHANG Jingyuan;CHEN Huanqiu;YAN Feng;CHEN Jun(Department of Interventional Radiology, Jiangsu Provincial Cancer Hospital, Affiliated Cancer Hospital of Nanjing Medical University, Nanjing, Jiangsu Province 210009, China)

机构地区：[1]江苏省肿瘤医院(南京医科大学附属肿瘤医院)介入科,南京210009 [2]河南省济源市中医院放射科 [3]江苏省肿瘤医院(南京医科大学附属肿瘤医院)普外科,南京210009 [4]江苏省肿瘤医院(南京医科大学附属肿瘤医院)病理科,南京210009 [5]江苏省肿瘤医院(南京医科大学附属肿瘤医院)检验科,南京210009

出　　处：《介入放射学杂志》2018年第6期544-548,共5页Journal of Interventional Radiology

基　　金：江苏省第四期"333工程"科研项目(BRA2015492);江苏省卫生计生委保健科研项目(bj14020);江苏省高层次卫生人才"六个一工程"拔尖人才科技项目(LGY2017087);江苏省科教强卫工程项目(CXTDA2017017)

摘　　要：目的 探讨经胃左动脉注入VX2瘤粒建立兔胃癌模型的可行性。方法 DSA导引下经股动脉将0.5 mm×0.5 mm VX2瘤粒注入18只新西兰大白兔胃左动脉。术后2、4d分别随机处死3只实验兔,获取胃大体标本并作病理学分析;术后7d剩余实验兔作胃左动脉DSA和食管胃消化道气钡双重造影,观察肿瘤生长情况,并即刻处死3只兔作病理学分析;余9只实验兔待其自然死亡,记录生存时间。结果 实验兔胃癌建模成功率为100%(18/18)。术后2、4d,大体标本可见胃底近大弯侧局部黏膜水肿、溃疡形成,镜下见局部黏膜缺失呈溃疡改变,黏膜下水肿明显,局部动脉血管内可见癌栓。术后7d剩余实验兔全部存活,胃左动脉DSA见胃底贲门处富血供病灶,染色明显;气钡双重造影见胃底贲门处黏膜紊乱,病灶区域见压迹及双边征;大体标本见胃底部病灶呈火山口样溃疡,周边黏膜明显充血、紊乱;镜下见肿瘤细胞聚集于肌层并向黏膜及浆膜层侵犯。剩余实验兔术后中位生存时间12d,大体标本见胃病灶穿孔,周边黏膜紊乱伴明显充血水肿;镜下见肿瘤侵犯胃壁全层。结论 DSA引导下经胃左动脉输注VX2瘤粒可成功建立兔胃癌模型,可用于介入动脉化疗栓塞、影像诊断及外科手术等相关实验研究。Objective To discuss the feasibility of injecting VX2 tumor particles through left gastric artery to establish the gastric cancer model in rabbits. Methods Guided by DSA, certain amount of 0.5× 0.5mm sized VX2 tumor particles was injected through left gastric artery via femoral artery access in 18 New Zealand rabbits. Every 3 randomly-selected experimental rabbits were sacrificed each time at 2 and 4 days after the procedure, the stomachs were removed and sent for pathological examination. DSA of [eft gastric artery and air-barium double-contrast radiography of esophagus and stomach were performed at 7 days after the procedure, and the tumor growth status was evaluated, meanwhile, 3 randomly-selected experimental rabbits were sacrificed for pathological analysis. The remaining 9 experimental rabbits were kept on feeding until they naturally died, and the survival time was recorded. Results The gastric cancer modeling success rate in experimental rabbits was 100% （18/18）. At 2 and 4 days after the procedure, on gross specimen local mucosal edema and ulceration could be observed at the gastric fundus near the greater curvature of the stomach. Microscopically, damaged local mucosa, presenting as ulceration, obvious submucosal edema, and cancer embolus in local arterial lumen could be seen. At 7 days after the procedure, the remaining experimental rabbits were all alive; DSA of left gastric artery showed that well-supplied lesions with obvious staining were displayed at gastric fundus cardia; air-barium double- contrast radiography of esophagus and stomach revealed that disordered mucosal folds at gastric fundus cardia, impression sign and double contour sign could be observed at the lesion area. Maeroscopically, the lesion at the gastric fundus presented as crater-like ulceration with the surrounding mucosa being obviously congested and disordered; microscopic examination showed that the tumor cells assembled in the muscular layer and both the mueosa and serosa were involved. The median survival time of the rem

关 键 词：胃癌 胃左动脉 放射学 介入性 兔模型 

分 类 号：R735.5[医药卫生—肿瘤]