miR-483-5p在肝细胞肝癌患者血清中的表达及临床意义  

Expresssion of miR-483-5p in serum of patients with hepatocellular carcinoma and it's clinical significance

作　　者：林延延[1] 张舟径[2] 曹洁[3] 白冰[1] 岳平[1] 把永江 孟文勃[1] LIN Yanyan;ZHANG Zhoujing;CAO Jie;BAI Bing;YUE Ping;BA Yongjiang;MENG Wenbo(Department of Special Minimally Invasive Surgery, The First Hospital of Lanzhou University, Lanzhou 730000, P. R. China;Department of Thyroicl Surgery, Ningbo No.2 Hospital, Ningbo, Zhejiang 315000, P. R. China;Clinical Laboratory, The First Hospital of Lanzhou University, Lanzhou 730000, P. R. China;The First Clinical College of Lanzhou University, Lanzhou 730000, P. R. China)

机构地区：[1]兰州大学第一医院特需外科,兰州730000 [2]宁波市第二医院甲状腺外科,浙江宁波315000 [3]兰州大学第一医院检验科,兰州730000 [4]兰州大学第一临床医学院,兰州730000

出　　处：《中国普外基础与临床杂志》2018年第6期689-694,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：甘肃省青年科技基金计划项目(项目编号:1606RJYA276);2017年兰州大学第一医院院内基金(项目编号:ldyyyn2017-04)

摘　　要：目的检测肝细胞肝癌(简称肝癌)患者血清中miR-483-5p水平并探讨其对肝癌的诊断价值。方法收集2010年1月至2012年1月期间兰州大学第一医院收治的112例肝癌患者术前1 d及术后30 d以及85例慢性乙型病毒性肝炎(简称慢性乙肝)患者和56例健康对照人群的清晨空腹血清样本。应用实时定量荧光PCR法检测血清中miR-483-5p和miR-500a水平,按常规电化学法检测血清中甲胎蛋白(AFP)水平,应用操作者工作特征(ROC)曲线分析血清中miR-483-5p、miR-500a及AFP水平用于肝癌的诊断价值。结果肝癌患者血清中miR-483-5p和miR-500a水平均明显高于慢性乙肝患者(P<0.000 1)和健康对照人群(P<0.000 1),但是其在慢性乙肝患者和健康对照人群间比较差异均无统计学意义(均P>0.05)。肝癌患者术后30 d时血清中miR-483-5p水平较术前明显下降(P<0.000 1)。miR-483-5p、miR-500a、AFP及miR-483-5p与AFP联合诊断肝癌的ROC曲线下面积(AUC)分别是0.74、0.66、0.81及0.92,当miR-483-5p临界值为2.842时的诊断灵敏度与特异度分别为74%和66%,当miR-500a的临界值为1.830时的诊断灵敏度和特异度分别是74%和51%,当AFP的临界值为20μg/L时的诊断灵敏度与特异度分别为78%和70%,当miR-483-5p与AFP联合使用的临界值为3.78时的诊断灵敏度和特异度分别为81%和83%。miR-483-5p诊断AFP阳性(AFP>20μg/L)和AFP阴性(0~20μg/L)肝癌患者的AUC值分别为0.78和0.83,二者的灵敏度和特异度分别为0.76、0.68和0.79、0.72。结论 miR-483-5p在肝癌患者的血清中呈高水平,其诊断肝癌有一定准确性,与AFP联合可以提高其诊断价值,并且其对AFP阴性肝癌患者的诊断具有重要的补充作用。Objective To detect expression of miR-483-5p in surem of patients with hepatocellular carcinoma （HCC） and investigate it＇s clinical significance for diagnosis of HCC. Methods The rerum samples of 112 patients with HCC （HCC group）, 85 patients with chronic viral hepatitis B （CHB group）, and 56 healthy people for physical examination （healthy control group） were collected from January 2010 to January 2012 in the First Hospital of Lanzhou University. According to the results of preliminary chip detection of miRCURY LNATM miRNA, the real-time fluorescent quantitative PCR was adopted to quantitate the serum levels of miR-483-5p and miR-500a and the routine electrochemical method was used to detect the serum alpha fetoprotein （AFP） in every group. The receiver operating characteristic （ROC） curve was utilized to analyze the diagnostic values of serum miR-483-5p, miR-500a, and AFP for the HCC. Results The serum levels of miR-483-5p and miR-500a in the HCC group were significantly higher than those of the CHB and healthy control groups （both P〈0.000 1）, which had no significant differences between the CHB group and the healthy control group （P〉0.05）. The serum miR-483-5p level of the HCC patient decreased markedly at the postoperative 30 d （P〈0.000 1） as compared with the preoperative level. The area under the ROC curve （AUC） of miR-483-5p, miR-500a, AFP, or miR-483-5p in combination with AFP for the diagnosis of the HCC was 0.74 （cutoff value=2.842, sensitivity=74% and specificity=66%）, 0.66 （cutoff value=1.830, sensitivity=74% and specificity=51%）, 0.81 （cutoff value=20 μg/L, sensitivity=78% and specificity=70%）, and 0.92 （cutoff value=3.78, sensitivity=81% and specificity=83%）, respectively. The AUC values of miR-483-5p in the diagnosis of the HCC patients with positive AFP （AFP〉20 μg/L） and negative AFP （0-20 μg/L） were 0.78 and 0.83, respectively. Conclusions Serum miR-483-5p highly expresses in HCC, which has a certain accuracy in diagnosi

关 键 词：MIRNA 标志物 肝细胞肝癌 miR-483-5p 甲胎蛋白 

分 类 号：R735.7[医药卫生—肿瘤]