半胱氨酰白三烯受体对小鼠小胶质细胞吞噬功能的调节作用  

Effects of cysteinyl leukotriene receptors on phagocytosis of mouse microglial cells

作　　者：王晓蓉 卢韵碧[2] 张纬萍[2] 魏尔清[2] 方三华[2,3] WANG Xiaorong;LU Yunbi;ZHANG Weiping;WEI Erqing;FANG Sanhua(Department of Pharmacy, Women＇s Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China;Department of Pharmacology, Zhejiang University School of Medicine, Hangzhou 310058, China;Core Facilities, Zhejiang University School of Medicine, Hangzhou 310058, China)

机构地区：[1]浙江大学医学院附属妇产科医院药剂科,浙江杭州310006 [2]浙江大学医学院药理学系,浙江杭州310058 [3]浙江大学医学院公共技术平台,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2018年第1期10-18,共9页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省自然科学基金(LY17H090009);浙江省公益技术应用研究(分析测试)计划项目(2016F82G2010036)

摘　　要：目的:研究半胱氨酰白三烯(CysLT)受体(CysLT_1R和CysLT_2R)对小鼠BV2小胶质细胞吞噬功能的调节作用。方法:以经典炎症激活剂脂多糖和CysLT受体激动剂LTD_4处理BV2细胞,采用免疫荧光计数法和流式细胞仪检测BV2细胞吞噬功能,免疫荧光共染法观察BV2细胞中CysLT_1R和CysLT_2R表达分布。结果:脂多糖和LTD_4均能增强BV2细胞吞噬功能,而CysLT_1受体选择性拮抗剂孟鲁司特和CysLT_2受体选择性拮抗剂HAMI 3379均能抑制脂多糖和LTD_4诱导的BV2细胞吞噬功能增强;脂多糖和LTD_4激活BV2细胞后可引起CysLT_1R和CysLT_2R在细胞内分布变化,两种亚型的受体分布变化趋势基本一致,且存在共表达。结论:CysLT_1R和CysLT_2R均可以调节BV2细胞的吞噬功能,且两者具有协同性。Objective：To determine the effects of cysteinyl leukotriene receptors（CysLT1R and CysLT2R）on phagocytosis of mouse BV2 microglial cells.Methods：BV2 cells were stimulated with microglial activators lipopolysaccharide（LPS）or CysLT receptor agonists LTD4.The phagocytosis of BV2 cells was observed by immunofluorescence analysis and flow cytometry.The intracellular distributions of CysLT1R and CysLT2R in BV2 cells were examined with immunofluorescence staining.Results：Both LPS and LTD4could significantly enhance the phagocytosis of BV2 cells,and such effect could be inhibited by CysLT1R selective antagonist Montelukast and CysLT2R selective antagonist HAMI 3379.The activation of BV2cells induced by LTD4or LPS resulted in changes in intracellular distributions of CysLT1R and CysLT2R.CysLT1R and CysLT2R was co-localization with a similar distribution.Conclusion：CysLT1R and CysLT2R regulate the phagocytosis of mouse BV2 microglial cells with a synergistic effect.

关 键 词：半胱氨酸/代谢 受体 白三烯/代谢 吞噬作用/药物作用 小神经胶质细胞/药物作用 脂多糖类/药理学 细胞 培养的 

分 类 号：R743[医药卫生—神经病学与精神病学] R96[医药卫生—临床医学]