胶东地区MSSA与MRSA的分离鉴定与耐药性分析  

Isolation and identification of drug resistant MRSA and MSSA from pigs of Jiaodong area

作　　者：王龙光 黄秀梅 逄春华 李玉清 刘焕奇 曲志娜 WANG Long-guang;HUANG Xiu-mei;PANG Chun-hua;LI Yu-qing;LIU Huan-qi;QU Zhi-na(College of Animal Science, Qingdao Agricultural University, Qingdao, Shandong 266109 ,China;China Animal Health and Epidemiology Center, Qingdao, Shandong 266032, Chinas;Qingdao Entry＆Exit Inspection and Quarantine Bureau, Qingdao, Shandong 266108, China)

机构地区：[1]青岛农业大学动物科技学院 [2]中国动物卫生与流行病学中心 [3]青岛机场出入境检验检疫局

出　　处：《中国兽医学报》2018年第6期1151-1156,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(31472237)

摘　　要：了解胶东地区猪源耐甲氧西林金黄色葡萄球菌（MRSA）的临床感染现状及耐药性，为临床合理使用抗生素类药物提供数据依据。利用多重PCR方法，扩增葡萄球菌属16S rRNA基因，金黄色葡萄球菌属nuc基因以及耐药基因mecA；血浆凝固酶试验对MRSA进行鉴定；微量肉汤稀释法对MRSA的耐药表型进行检测；运用SPSS20．0软件对试验数据进行统计分析。结果显示：共分离出508株金黄色葡萄球菌，其中MRSA122株，占24．02％；甲氧西林敏感金黄色葡萄球菌（MSSA）386株，占75．98％。所有样品均来自健康猪只的鼻腔拭子和体表拭子。MRsA对AM和P的耐药率为100％；对CLI、CEF、TIL、CZ、EM、A／C和SXT的耐药率均在80％以上；对OFL、ENR和SF的耐药率较低，分别为13．11％，5．74％和4．10％。MSSA对EM、AM和P的耐药率均在90％以上；对CLI、CEF、TIL、CZ和SXT的耐药率均在50％以上；对ENR、OFL和AjC的耐药率分别为25．65％，47．15％，41．71％；对SF的耐药率最低为2．33％。MRSA与MSSA未检出耐VAN的菌株。MRSA的多重耐药率为100％，多集中在7耐及以上；MSSA多重耐药率为99．22％，多集中在3～11耐。结果表明，MRSA的检出率较高，MRSA的耐药率及多重耐药率高于MSSA，且均处于较高水平，应给予重视，临床应依据药敏试验结果合理选择抗茵类药物。To analyze the incidence and resistant of methicillin-resistant staphylococcus aureus（MRSA） so as to provide evidence for the reasonable clincial use of antibiotics. The Staphylococcus gene 16S rRNA,Staphylococcus aureus gene nuc and methicillin resistance gene m ecA were detected by multiple polymerase chain reaction and the plasma coagulase tests for separation and identification,micro broth dilution method was used to detect drug-resistance phenotype. And SPSS20.0 software was employed to analyze the experimental data. Results showed that a total of 508 strains of S, aureus from were collected the samples,incluing 122（24.02%）MRSA strains,386（75.98%） MSSA strains. All samples were from nasal swabs and surface swabs from healthy pigs. The resistance rate of MRSA to AM and P were 100%,to CLI,CEF,TIL,CZ,EM,A/C,SXT were more than 80%;to OFL, ENR, SF were 13. 11%, 5. 74 %, 4. 10%, respectively. The resistance rate of MSSA to EM,AM and P were more than 90% ;CLI,CEF,TIL,CZ,SXT were more than 50%,to ENR,OFL,A/C were 25. 65%,47.15%,41.71%,to SF was the lowest （2.33%）. There was no resistant strains was to VAN detected. The multidrug resistance rate of MRSA was 100% ,and the resistance drugs were 7 or above;the multidrug resistance rate of MSSA was 99.22%,the resistance drugs were the 3 to 11. It is concluded that the rate of MRSA detection and multiple drug resistance rates were more than thoes of MSSA,and both were at a high level. It is necessary to reasonably use antibiotics based on the result of drug susceptibility testing.

关 键 词：MRSA MSSA 猪源 分离鉴定 耐药性 

分 类 号：S855.11[农业科学—临床兽医学] R535[农业科学—兽医学]