草鱼烂鳃病病原约氏黄杆菌的分离与鉴定及组织病理观察  

Isolation, identification of a Flavobacteriumjohnsoniae from the Bacterial Gill Rot Disease of Grass Carp and Histopathological Observation

作　　者：刘礼辉[1] 付小哲[1] 林强[1] 梁红茹[1] 李宁求[1] LIU Lihui;FU Xiaozhe;LIN qiang;LIANG Hongru;LI Ningqiu(Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key Laboratory of Aquatic Animal lmmune Technology, Guangzhou, 510380, China)

机构地区：[1]中国水产科学研究院珠江水产研究所,农业部渔用药物创制重点实验室,广东省水产动物免疫技术重点实验室,广东广州510380

出　　处：《珠江水产科学》2018年第2期76-82,共7页Finsheries Science and Technology Industry

基　　金：中国水产科学研究院基本科研业务费专项,2016GH09;2017年度广东省渔港建设和渔业发展专项资金（鱼病防治）项目

摘　　要：本研究从患病的草鱼鳃上分离到菌株GQYGCG0711，经回归感染试验证明是草鱼细菌性烂鳃病的病原菌。经形态观察和生理生化分析、16SrRNA序列同源性比较以及系统发育分析，鉴定该菌株为约氏黄杆菌（Flavobacterium johnsoniae）。药敏试验结果表明，该菌对对妥布霉素和多粘菌素B不敏感。对发病草鱼肝和鳃进行了病理组织切片电镜观察，结果显示约氏黄杆菌附着在鳃上皮细胞上，鳃小片上皮细胞明显增生，上皮细胞空泡化，鳃丝之间的上皮融合，鳃小片的远端边缘互相融合，完全被覆没，并发生腐脱和崩解，中心软骨结构坏死；肝细胞有变性和肿大。A bacterial strain named GQYGCG0711 was separated and sublimated from the bacterial gill rot disease of grass carp, and confirmed to be pathogenic strain by recursive infection experiments. The strain was identified as Flavobacterium johnsoniae from morphological examination physiological and biochemical characteristics as well as 16S rRNA sequence and phylogenetic analysis. Drug susceptibility test showed that this strain was resistant to tobramycin and olymixin B. The structures of gill and liver of carp were investigated using scanning electron microscopy. The results showed that the strain was attached on the gill epithelial cells, damage to epidemic cell included hyperplasia, hypertrophy and vacuolization. The distal edge of gill lamellae were fusion each other, and desquamation, the center structure of cartilage was necrosis. The hypertrophied cells and degeneration of cells were located in the cells of liver.

关 键 词：烂鳃病 约氏黄杆菌 16S RRNA 药敏 病理观察 

分 类 号：S942[农业科学—水产养殖]