猪繁殖与呼吸综合征病毒Nsp9基因第642位氨基酸突变对病毒复制的影响  

Influence of 642 amino acid mutations of Nsp9 gene to replication of PRRSV

作　　者：李华玮[1] 姬鹏超 王永芬[1] 赵绪永[1] 何健[2] 赵孟孟[2] LI Hua-wei;JI Peng-chao;WANG Yong-fen;ZHAO Xu-yong;HE Jian;ZHAO Meng-meng(Bioengineering department , Henan University of Animal Husbandry and Economy, Zhengzhou 450046 , China;College of Veterinary Medicine and Animal Science , Henan Agricultural University , Zhengzhou 450002 , China)

机构地区：[1]河南牧业经济学院生物工程学院,河南郑州450046 [2]河南农业大学牧医工程学院,河南郑州450002

出　　处：《中国兽医杂志》2018年第3期11-14,19,共5页Chinese Journal of Veterinary Medicine

基　　金：河南省高等学校重点科研项目(15A230030);十二五"农村领域国家科技计划课题:高致病性蓝耳病等重要家畜疫病新型疫苗研究与开发(2015BAD12B02);河南省科技攻关项目(162102110033);河南牧业经济学院科技创新团队项目(HUAHE2005001)

摘　　要：为了探索Nsp9基因上与病毒复制相关的关键氨基酸位点,通过融合PCR方法突变第642位氨基酸,之后进行病毒的拯救,并对病毒的滴度进行测定。结果表明,突变第642位氨基酸之后可以成功拯救病毒,突变毒株的滴度与亲本毒株的滴度未有明显差异,但突变毒株的转录水平有所下降。初步得出结论:猪繁殖与呼吸综合征病毒Nsp9基因第642位氨基酸与病毒滴度无关,与病毒转录相关。To explore the key amino acid sites associated with viral replication in Nsp9. The 642 amino acid site was mutated by using the overlap PCR method,and then the virus was rescued using the basis reverse genetics and the titers were also analyzed. The virus was successfully rescued. Titers of the rescued virus was not significantly varied from that of the parental strain XH-GD,but the Nsp9 mRNA transcription level was lower. Our preliminary results show that 642 amino acid site in Nsp9 has no influence on the titer of PRRSV,but is related to Nsp9 mRNA transcription.

关 键 词：猪繁殖与呼吸综合征病毒 Nsp9基因 反向遗传学 病毒拯救 642位氨基酸 

分 类 号：S823.91[农业科学—畜牧学]