微小RNA-203在乳腺导管增生性疾病中表达及其临床意义  

Detect the microRNA -203 gene protein expression in mammary gland lesions and their relation-ships with clinical indicators

作　　者：张娜 孙华朋[2] 廖晓锋[2] Zhang Na;Sun Huapeng;Liao Xiaofeng(Department of Pathology, Xiangyang Central Hospital, Hubei College of Liberal Arts and Sciences, Xiangy- ang 441021, China;Department of General Surgery, Xiangyang Central Hospital, Hubei College of Liberal Arts and Sciences, Xiangyang 441021, China)

机构地区：[1]襄阳市中心医院湖北文理学院附属医院病理科,441021 [2]襄阳市中心医院湖北文理学院附属医院普外科,441021

出　　处：《中华实验外科杂志》2018年第6期1030-1033,共4页Chinese Journal of Experimental Surgery

摘　　要：目的检测微小RNA（miRNA，miR）-203mRNA在乳腺浸润性导管癌（IDC）及增生性病变中的表达，探讨在各病变中的作用和临床病理间的关系。方法应用一步法反转录一聚合酶链反应（RT-PCR）检测IDC及癌旁组织各60例及99例乳腺增生性病变和22例正常乳腺新鲜标本中miR-203mRNA表达；并结合临床病理特征分析。结果miR-203mRNA表达强度在正常乳腺组织、导管上皮普通性增生（UDH）、导管上皮非典型增生（ADH）和导管原位癌（DCIS）及IDC中分别为1．36、0．98、0．79、0．66和0．42，逐渐减弱且差异有统计学意义（P=0．005）。RT-PCR结果显示miR-203mRNA：（1）在IDC60例和癌旁组织中的表达水平分别为12％、89％，差异有统计学意义（P=0．003）；（2）区域淋巴结转移：表达强度显示阳性组显著低于阴性组，NO〉N1〉N2期（P=0．000）；（3）临床TNM分期：表达强度两两比较显示Ⅰ期〉Ⅲ期（P=0．000）；（4）组织学分级中：表达强度Ⅰ级〉Ⅱ级〉Ⅲ级（P=0．000）。（5）雌激素受体（ER）、孕激素受体（PR）和人表皮生长因子受体2（C-erbB-2）表达的关系：表达强度在ER、PR、C-erbB-2（-）与ER、PR、C-erbB-2（＋＋）、（ ）比较，差异有统计学意义（P=0．003）。结论在IDC和不同乳腺增生性组织中其蛋白表达强度存在差异。miR-203基因异常表达在IDC发生发展过程中起重要的调控作用。Objective To detect the microRNA （miRNA, miR） -203 mRNA expression in inva- sive ductal carcinoma of the breast （ IDC ） and adjacent non - cancerous lesions, and their relationships with clinical indicators of IDC and hyperplasia lesions were analyzed. Methods MiR - 203 mRNA of 60 breast IDC and 60 normal breast tissues and 99 breast hyperplasia lesions and 22 normal breast tissues were amplified by one -step reverse transcriptase -polymerase chain reaction （RT- PCR） method. Results RT - PCR showed miR - 203 mRNA ： （ 1 ） The standardized coefficient were 1.36, 0. 98, 0. 79, 0. 66 and 0. 42, which were gradually lower in normal breast tissues, UDH, ADH, DCIS and IDC （P = 0. 005 ）. The expression rate and standardized coefficient in IDC were significantly lower than those of breast tissues （ P = 0. 003 ）. （2） The standardized coefficient in cases with axillary node metastasis was sig- nificantly lower than that in cases without axillary node matastasis, NO 〉 N1 〉 N2 （P = 0. 000）. （3）The standardized coefficient at stage I was significantly higher than that at stage Ⅱ , and stage Ⅱ was signifi- cantly higher than stage Ⅱ （ P = 0. 000 ）. （ 4 ） The standardized coefficient at grade I was significantly higher than that at grade Ⅱ , and grade 11 was significantly higher than grade m （ P = o. 000 ）. （ 5 ） The standardized coefficient at estrogen receptor （ ER）, progesterone receptor （PR） , C - erbB - 2 （ - ） was significantly higher than ER, PR, C - erbB - 2 （ ） , （ ） （ P = 0. 003 ）. Conclusion The miR -203 protein expression in IDC and hyperplasia lesions have different levels, miR -203 may play a important role in IDC.

关 键 词：微小RNA-203 乳腺浸润性导管癌 乳腺增生性病变 

分 类 号：R737.9[医药卫生—肿瘤]