脂肪酸合成酶抑制剂浅蓝菌素对裸鼠膀胱癌移植瘤生长抑制的作用  

Research of fatty acid synthase complex inhibitors cer on growth inhibition in human bladder canc-er nude mice transplanted tumor

作　　者：仇世钦 刘经纬 孙旭杰 朱永 梁君峰 高德荣 郑帅帅 姜波[2] 宋士强 Qiu Shiqin;Liu Jingwei;Sun Xujie;Zhu Yong;Liang Junfeng;Gao Derong;Zheng Shuaishuai;Jiang Bo;Song Shiqiang(Department of Urology, People＇ s Hospital of Chenyang District in Qingdao, QingDao 266109, China;Department of Urology, Municipal Hospital of Qingdao, QingDao 266071, China)

机构地区：[1]青岛市城阳区人民医院泌尿外科,266109 [2]青岛市市立医院泌尿外科,266071

出　　处：《中华实验外科杂志》2018年第6期1097-1099,共3页Chinese Journal of Experimental Surgery

摘　　要：目的观察应用浅蓝菌素（Cer）抑制脂肪酸合成酶（FASN）对人膀胱癌裸鼠移植瘤生长的影响。方法建立膀胱癌细胞5637裸鼠皮下移植瘤模型，将成瘤的20只小裸鼠随机分成阴性对照组、Cer治疗组，观察并记录两组裸鼠体重及移植瘤的体积的变化。免疫组织化学法检测各组裸鼠皮下移植瘤中细胞核增殖抗原（Ki-67）的表达；脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法（TUNEL）法检测移植瘤组织的细胞凋亡水平。结果随机分组的裸鼠分别应用Cer和二甲基亚砜（DMSO）处理后体重变化差异无统计学意义；Cer治疗组裸鼠移植瘤的体积较DMSO对照组明显减小（P=0．023）；Ki-67的表达较对照组明显减少（P=0．034），Cer治疗组的细胞凋亡水平明显升高（P=0．026）。结论FASN抑制剂Cer可促进裸鼠膀胱癌移植瘤细胞的凋亡并且对移植瘤生长有明显的抑制作用。Objective To investigate the application of cerulenin （cer） inhibiting fatty acid synthase complex （FASN） on human bladder cancer nude mice transplanted tumor growth. Methods Human bladder cancer nude mice transplanted tumor models were established, and 20 nude mice were randomly divided into 2 groups ： the control group and the cer group. Observe and record the change of weight and the volume of the transplanted tumor. The expression of proliferation cell nuclear antigen （ Ki - 67 ） was deter- mined by immunocytochemistry （IC）. Apoptosis levels of transplanted tumor were detected by TdT - medi- ated dUTP nick end labeling （TUNEL） assays. Results Random grouping of the nude mice respectively the Cer and Dimethylsulfoxide （DMSO） weight change has no significantly difference after the treatment~ Tumour volumes in the experimental group were significantly smaller than those in the control group （ P = 0. 023 ） , the expression of Ki - 67 were significantly lower than those in the control group （ P = 0. 034 ） , the apoptotic indexes were significantly higher than the control group （P = 0. 026 ）. Conclusion FASN inhibitors Cer can promote nude mice transplantation tumor cell apoptosis and has significantly inhibitory effect on transplanted tumor growth.

关 键 词：脂肪酸合成酶 浅蓝菌素 膀胱癌 细胞核增殖抗原 凋亡 裸鼠 

分 类 号：R737.14[医药卫生—肿瘤]