甲强龙对脑出血后灶周MMP-9表达及其神经损伤的保护作用  

The study of the post-hemorrhagic neuroprotective effect of methylprednisolone on intracerebral hemorrhage in rats

作　　者：毛珂[1] 雷町[1] 游潮[1] 肖安琪[1] 郑松平 陈锐奇 任艳明[1] MAO Ke;LEI Ding;YOU Chao;XIAO Anqi;ZHENG Songping;CHEN Ruiqi;REN Yanming(Department of Neurosurgery , West China Hospital, Sichuan University, Chengdu 610041, China)

机构地区：[1]四川大学华西医院神经外科,四川成都610041

出　　处：《西部医学》2018年第6期789-793,共5页Medical Journal of West China

基　　金：四川省卫生和计划生育委员会科研项目(17PJ133)

摘　　要：目的探讨甲强龙对脑出血后灶周基质金属蛋白酶-9(MMP-9)表达及神经损伤保护作用的实验研究。方法通过立体定向仪建立稳定的SD大鼠高血压脑出血模型,将实验动物30只随机分为假手术对照组、脑出血组、甲强龙干预组、空白对照组,每组各7只。甲强龙干预组于术后1小时及24小时后给予35mg/kg的甲强龙腹腔注射,空白对照组腹腔注射等量的生理盐水。在术后第1、3、7天检测各组大鼠的脑含水量、神经功能评分及MMP-9蛋白表达;在术后第3天检测各组大鼠的细胞凋亡、活化小胶质细胞数量。结果甲强龙干预组术后第1、3、7天的脑组织含水量明显低于及空白对照组,组间比较差异有统计学意义(P<0.05);甲强龙干预组术后第1、3、7天的神经功能评分明显高于空白对照组,组间比较差异有统计学意义(P<0.05);术后第3天的OX-42免疫组化染色显示甲强龙干预组活化小胶质细胞数量少于空白对照组,组间比较差异有统计学意义(P<0.05);甲强龙干预组术后的MMP-9蛋白表达明显低于空白对照组,组间比较差异有统计学意义(P<0.05)。结论甲强龙能抑制大鼠脑出血后MMP-9的高表达且能减轻脑水肿和炎性反应,具有神经功能保护作用。Objective To establish a stable intracerebral hemorrhagic model in SD rats, and analyze the neuroprotective effect of methylprednisolone on intracerebral hemorrhagic model by studying the cerebral edema, neuro-functional scores, the postoperative OX-42 immunohistochemical staining and MMP-9 protein expression on the hypertensive rats. Methods The SD rat model of hypertensive cerebral hemorrhage was estalished by stereotactic apparatus. The rats were divided into sham operation group,cerebral hemorrhage group, methylprednisolone group and control group. Methylpred- nisolone group was treated with intraperitoneal injection of methylprednisolone （35mg/kg）. The control group was injected with same measure saline. The cerebral water content, neurological behavioral scores, MMP-9 protein expression, apoptosis and activation of microglia in rats were observed. Results The cerebral water content of SHRs in methylprednisolone group were significantly lower than that in control group at different time points （P〈0.05）. The neurological behavioral scores in methylprednisolone group were significantly lower than that in control group at different time points （P〈0.05）. The postoperative OX-42 immunohistochemical staining on 3rd day showed the number of active microglia in methylprednisolone group were less thanthat in the control group （P〈0.05）. MMP-9 expression of the brain tissue surrounding the hematoma in methylprednisolone group was lower than the control group （P〈0.05）. Conclusion Methylprednisolone could inhibit MMP-9 expression after intracerebral hemorrhage. Methylprednisolone has neuroprotective effect in terms of reducing cerebral edema, inflammatory response.

关 键 词：脑出血 甲强龙 神经保护作用 MMP-9 

分 类 号：R743.34[医药卫生—神经病学与精神病学]