基于“肾应冬”理论探讨季节变化对金黄地鼠免疫系统的影响  

Discussion on the Effect of Seasonal Change on Golden Hamsters' Immune System Based on the Theory of “Kidney Corresponds with Winter”

作　　者：李雨欣 许筱颖 郭霞珍 施娜 Li Yuxin;Xu Xiaoying;Guo Xiazhen;Shi Na(School of Traditional Chinese Medicine, Beijing University of Traditional Chinese Medicine, Beijing 100029, China)

机构地区：[1]北京中医药大学中医学院,北京100029

出　　处：《世界中医药》2018年第4期944-948,共5页World Chinese Medicine

基　　金：国家自然科学基金项目(81573840)

摘　　要：目的:观察松果体摘除术金黄地鼠白介素-1(Interleukin-1,IL-1)、IL-2、IL-6及IgE、IgG在四季中的水平并与正常金黄地鼠进行比较,探讨松果体对免疫系统的季节性调控机制,揭示"肾应冬"的内涵。方法:选取2个月龄金黄地鼠,分为春分、夏至、秋分、冬至4个节气组,每个节气组内又分实验组(行松果体摘除术)、对照组。动物饲养至春分、夏至、秋分、冬至4个时间点当日,于晚9:00后腹主动脉取血,用酶联免疫吸附试验测定相关免疫指标。结果:IL-2、IL-6、IgE、IgG的水平变化具有明显的季节节律性;IL-1、IL-2、IL-6、IgG水平在冬季达到最低;实验组和对照组间比较,显示松果体能调控IL-2、IgE、IgG的分泌,IL-2在被摘除松果体后失去了季节变化的规律性。结论:血清白介素和免疫球蛋白水平基本存在春夏高、秋冬低的季节性变化规律,这与中医"冬季封藏"的理论不谋而合,且松果体在此过程中对免疫系统发挥部分调节作用。Objective： To discuss the effect of the pineal in modulating the immune system and the connotation of ＂kidney corresponds with winter＂through observing the level of golden hamster＇ s interleukin-1（ IL-1）,IL-2,IL-6 and IgE and IgG in seasons after removing the pineal and comparing it with normal golden hamsters. Methods： Two-month-old Golden hamsters were divided into four groups： vernal equinox,summer solstice,autumn equinox and winter solstice. Each group was subdivided into experimental group（ row pineal extirpation） and control group. Golden hamsters were fed until vernal equinox,summer solstice,autumnal equinox and winter solstice. Blood was drawn from abdominal aorta on these four days after 9： 00 pm,and ELISA method was used to measure the relevant immune index. Results： The content of IL-2,IL-6,IgE and IgG had obvious seasonal rhythmicity. The content of IL-1,IL-2,IL-6 and IgG reached the lowest in winter. It was shown that the pineal could regulate the secretion of IL-2,IgE and IgG,and IL-2 lost the regularity of seasonal change after removing the pineal. Conclusion： The content of serum interleukin and immune globulin basically is high in spring and summer and low in autumn and winter,which coincides with the theory of＂winter-storing＂in traditional Chinese medicine. And the pineal can partly regulate the immune system.

关 键 词：肾应冬 二分二至 季节变化 金黄地鼠 免疫系统 白介素 免疫球蛋白 松果体 

分 类 号：R229[医药卫生—中医基础理论]