植物激素及维生素对微藻GN6生长及油脂产量的影响研究  

Effect of phytohormones and vitamins on growth and oil production of microalgae GN6

作　　者：李汉广[1] 周秋香[2] 杨治友 黎秋玲 张庆华[1] LI Hanguang;ZHOU Qiuxiang;YANG Zhiyou;LI Qiuling;ZHANG Qinghua(Jiangxi Key Laboratory for Conservation and Utilization of Fungal Resources, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources, College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang 330045, China;Affiliated Hospital of Jiangxi Agricultural University, Nanchang 330045, China)

机构地区：[1]江西农业大学生物科学与工程学院,江西农业微生物资源开发与利用工程实验室,江西省茵物资源保护与利用重点实验室,南昌330045 [2]江西农业大学医院,南昌330045

出　　处：《中国油脂》2018年第6期115-119,共5页China Oils and Fats

基　　金：国家自然科学基金项目(21466014);江西省教育厅科学研究项目(GJJ160388)

摘　　要：以自行筛选的微藻GN6为试验菌种,首先研究海绿素、复硝酚钠、吲哚乙酸3种植物激素及维生素B1和维生素B12两种维生素对微藻生长及油脂产量的影响,然后在上述单因素试验研究结果基础上进行了四因素三水平的正交试验优化。结果表明:微藻GN6以BG11为基础培养基的最佳培养条件为1.5 m L/L海绿素、15μg/L维生素B12、0.60 g/L蛋白胨、20 g/L葡萄糖;将优化后的培养条件和未优化的培养条件进行比较,结果发现在最优条件下微藻GN6的生物量及油脂产量分别为16.4 g/L和1.62 g/L,与未优化相比分别提高了100.3%和52.2%。试验为通过添加植物激素或维生素提高产油脂微藻的生长及产油脂能力提供了有益的探索。With self - screened microalgae GN6 as experimental strain, the effects of phytohormones such as seaweed liquid fertilizer, sodium nitrophenolate, and indol -3 -acetic acid and vitamins such as vitamin BI and vitamin Blzon the growth and oil production of microalgae GN6 were researched. Based on single factor experiment, four factors and three levels orthogonal experiment was used to optimize the cul- ture conditions. The results showed that with BG11 as the basic culture medium, the optimal culture conditions of microalgae GN6 were obtained as follows：l. 5 mL/L seaweed liquid fertilizer, 15 pLg/L vitamin Bl2, 0.60 g/L peptone and 20 g/L glucose. Under the optimal conditions, the biomass and oil productivity were 16.4 g/L and 1.62 g/L, respectively, which were 100.3% and 52.2% higher than those before optimization. Therefore, these results could provide a beneficial exploration for the further improvement of growth and oil production of oil-rich microalgae by adding phytohormones and vitamins.

关 键 词：微藻GN6 生物量 油脂产量 植物激素 维生素 

分 类 号：S216.2[农业科学—农业机械化工程] Q949.9[农业科学—农业工程]