基于mRNA-Seq的沙漠植物花花柴干旱胁迫表达谱分析  

Analysis of expression profile in Karelinia caspia seedling under drought stress based on mRNA-Seq

作　　者：王彦芹[1,2] 沈李丽 罗来鑫[3] 李健强[3] 李志军[1,2] WANG Yan-Qin;SHEN Li-Li;LUO Lai-Xin;LI Jian-Qiang;LI Zhi-Jun(The Xinjiang Production and Construction Corps Tarim Basin Key Laboratory of Biological Resources Protection, Alar, Xinjiang 843300, China;College of Life Sciences, Tarim University, Alar, Xinjiang 843300, China;The Seed Pathology Laboratory of China Agricultural University, Beijing 100193, China)

机构地区：[1]新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300 [2]塔里木大学生命科学学院,新疆阿拉尔843300 [3]中国农业大学种子病理实验室,北京100193

出　　处：《植物生理学报》2018年第5期879-885,共7页Plant Physiology Journal

基　　金：新疆生产建设兵团科技计划项目(2012BB045);国家自然科学基金(31460071和31660085)~~

摘　　要：以抗逆性较强的荒漠植物花花柴为材料,利用数字基因表达谱技术对用质量百分数20%的PEG溶液处理0、4、8、12和24 h后的花花柴幼苗的m RNA-Seq测序结果进行分析。结果表明,花花柴幼苗叶部在不同处理时间均上调表达的基因有122个,下调表达的基因54个,根部在不同处理时间均上调表达的基因73个,下调表达的基因79个。利用q RT-PCR和RT-PCR对可能与花花柴耐旱相关的6个差异表达基因进行了验证,发现其表达模式与表达谱结果一致,推测这些基因可能均与花花柴响应干旱胁迫相关。本研究为耐旱相关基因的发掘及应用提供了基础。In this study, the gene expression profile of Karelinia caspica seedling was used for analysis under treatment with 20% PEG at 0, 4, 8, 12, and 24 h through mRNA-Seq technology. The results indicated that there were 122 genes up-regulated and 54 genes down-regulated expression in the leaves, and 73 genes up-regulated and 79 genes down-regulated expression in the roots of Karelinia caspica seedling under different time treatments. And then, 6 genes with differential expression were tested by qRT-PCR and RT-PCR. The result showed that the expression pattern of these 6 genes were as same as the mRNA-Seq. It was inferred that these genes might be related with drought stress in Karelinia caspica. This research provides a basis for the exploration and application of drought related genes from Karelinia caspica.

关 键 词：沙漠植物 花花柴 干旱胁迫 MRNA-SEQ 耐旱相关基因 

分 类 号：Q943.2[生物学—植物学]