小鼠实验性自身免疫性脑脊髓炎模型中CD4^＋T细胞表面CDld分子对多发性硬化疾病进展的影响  

Effect of CD4^＋ T cell surface CDld molecules on progression of multiple sclerosis in mouse experimental autoimmune encephalomyelitis model

作　　者：王宽红[1] 臧卫周[2] 李艳丽[1] 周俐红[1] Wang Kuanhong;Zang Weizhou;Li Yanli;Zhou Lihong(Department of Neurology, Xinxiang Central Hospital of Henan, Xinxiang 453000, Chin)

机构地区：[1]河南省新乡市中心医院神经内科,453000 [2]河南省人民医院神经内科

出　　处：《中华医学杂志》2018年第23期1873-1875,共3页National Medical Journal of China

摘　　要：目的探讨CD4^＋T细胞表面CDld分子对实验性自身免疫性脑脊髓炎（EAE）小鼠模型疾病进展的影响。方法建立C57BL／6小鼠的EAE模型，在疾病进展的不同时期分离小鼠脾细胞，用流式细胞术检测活化与非活化CD4^＋T细胞表面的CDld＋细胞比例。结果对照组（正常组和CFA组）、EAE组发病高峰期以及疾病恢复期相比较，对照组表达CDld分子的CD4^＋T细胞数量百分率为（8．98±0．36）％，EAE组高峰期及恢复期表达CDld分子的CD4^＋T细胞数量百分率分别为（2．14±O．15）％、（13．80±0．84）％，差异有统计学意义（P〈0．05）。结论验证了EAE发病过程中表达CDld分子的CD4^＋T细胞数量的变化趋势，为进一步研究EAE模型病程进展中CD4^＋T细胞和NKT细胞之间的相互作用奠定基础。Objective To investigate the effect of CD1 d molecules on the surface of CD4 ^＋ T cells on the progression of experimental autoimmune encephalomyelitis （ experimental, allergic, encephalomyelitis, EAE） mouse models. Methods EAE model of C57BL/6 mice was established, Splenic cells were isolated at different stages of the progression of the disease. The proportion of CD1 d^＋ cells on the surface of activated and non activated CD4^＋ T cells was detected by flow cytometry. Results The proportion of CD1 d^＋ cells in the control group （normal group and complete Freund＇s adjuvant （CFA） group）,in the peak and recovery period of disease in the EAE group were compared. The proportion of CD1 d^＋ cells in the control group was （ 8. 98 ＋ 0. 36） %, and the proportion of CD1 d^＋ cells in the peak and recovery period of disease in the EAE group were respectively （ 2. 14± 0. 15 ） % and （ 13.80 ± 0. 84 ） % . The differences were statistically significant difference （ P 〈 0. 05 ）. Conclusion The trend of the proportion of CD4^＋ T cells expressing CDld molecules during the course of EAE pathogenesis is verified, which lays a foundation for further study on the interaction between CD4^＋ T cells and NKT cells in the progression of EAE models.

关 键 词：实验性自身免疫性脑脊髓炎 CDld CD4^+T细胞 

分 类 号：R744.51[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]