Caspase-1在大鼠缺氧相关性肺高压中的作用及茶多酚对其影响的研究  

Effects of tea polyphenols on Caspase-1 in pulmonary tissue of rats with pulmonary hypertension induced by hypoxia

作　　者：雷敏[1] 徐戈[2] 黄琛[3] 欧婷[2] 王利兵[2] LEI Min;XU Ge;HUANG Chen(Department of Cardiovascular, the Red Cross Hospital of Yulin, Yulin 537000, Chin)

机构地区：[1]广西玉林市红十字会医院心内科,广西壮族自治区玉林市537000 [2]广西医科大学附属第一医院心内科 [3]广西中医药大学第一附属医院干保科

出　　处：《中国心血管病研究》2018年第5期468-471,I0001,共5页Chinese Journal of Cardiovascular Research

基　　金：广西自然科学基金资助项目(项目编号:2010GXNSFB013074)

摘　　要：目的 观察Caspase-1在大鼠缺氧相关性肺高压中的作用及茶多酚对Caspase-1的影响,探讨茶多酚防治缺氧相关性肺高压的可能机制.方法 将24只实验大鼠随机分为对照组（n=8）、肺高压组（n=8）和茶多酚组（n=8）.肺高压组和茶多酚组建立肺高压模型,茶多酚组加用茶多酚200 mg·kg-1·d-1干预.4周后测量大鼠平均肺动脉压（mPAP）,采用ELISA法检测血浆hs-CRP、IL-1β、Caspase-1浓度,RT-PCR检测肺组织Caspase-1 mRNA表达,Western blot检测肺组织Caspase-1蛋白表达.结果 肺高压组大鼠mPAP[（25.50±3.02）mm Hg]、血浆hs-CRP[（70.01±12.39）ng/L] 、IL-1β[（91.71 ±16.04）ng/L]、Caspase-1浓度[（134.06±16.03）ng/L]、肺组织Caspase-1 mRNA（1.239±0.304）和Caspase-1蛋白表达（0.329±0.051）均较对照组mPAP[（15.13± 1.96）mm Hg]、血浆hs-CRP[（20.68±4.29）ng/L]、IL-1β[（24.36±6.07）ng/L]、Caspase-1浓度[（31.64±5.41）ng/L]、肺组织Caspase-1 mRNA （0.708±0.138）和Caspase-1蛋白表达（0.118±0.015）明显升高（P均＜0.01）.茶多酚组mPAP[（20.50±0.93）mm Hg]、血浆hs-CRP[（49.16±9.02）ng/L]、IL-1β[（62.49± 17.03）ng/L]、Caspase-1浓度[（78.40±17.07）ng/L]、肺组织Caspase-1 mRNA（0.851±0.190）和Caspase-1蛋白表达（0.160±0.038）均较肺高压组明显降低（P均＜0.01）.相关分析显示,血浆Caspase-1与hs-CRP（r=0.784,P=0.000）和IL-1β（r=0.857,P=0.000）均呈正相关,肺组织Caspase-1蛋白表达量与mPAP呈正相关（r=0.809,P=0.000）.结论 Caspase-1通过调控炎症介质功能参与大鼠缺氧相关性肺高压的形成.通过降低血浆Caspase-1浓度和下调肺组织Caspase-1表达,进而抑制肺血管炎症,可能是茶多酚改善缺氧相关性肺高压的作用机制之一.Objective To investigate the role of Caspase-1 in rats with pulmonary hypertension induced by hypoxia and the effects of tea polyphenols on Caspase-1.Methods 24 male Sprague Dawley rats were divided into 3 groups：control group（n=8）,pulmonary hypertension group（n=8） and tea polyphenols group（n=8）.Rats in the tea polyphenols group were treated with tea polyphenols （200 mg·kg-1·d-1）.The rats were feed for 4 weeks.Then the mean pulmonary artery pressure（mPAP）,the levels of hs-CRP,IL-1β and Caspase-1 in blood plasma,the expressions of Caspase-1 in pulmonary tissue were detected.Results In control group,the mPAP was （15.13±1.96）mm Hg;the levels of hs-CRP,IL-1βand Caspase-1 in blood plasma were （20.68±4.29）ng/L,（24.36±6.07）ng/L and （31.64±5.41）ng/L;the expressions of Caspase-1 mRNA and protein were （0.708±0.138）and （0.118±0.015）.Compared with control group,mPAP （25.50±3.02）mm Hg,the levels of hs-CRP （70.01±12.39）ng/L,IL-1β（91.71±16.04）ng/L and Caspase-1 in blood plasma（134.06±16.03）ng/L,the expressions of Caspase-1 mRNA （1.239±0.304） and protein （0.329±0.051） were significantly increased in pulmonary hypertension group （all P〈0.01）.The mPAP （20.50±0.93）mm Hg,the levels of hs-CRP （49.16±9.02）ng/L,IL-1β（62.49±17.03）ng/L and Caspase-1 （78.40±17.07）ng/L in blood plasma,the expressions of Caspase-1 mRNA （0.851±0.190） and protein （0.160±0.038） in tea polyphenols group were significantly decreased than those in pulmonary hypertension group（all P〈0.01）.The level of Caspase-1 was positively correlated with the level of hs-CRP （r=0.784,P=0.000） and IL-1β（r=0.857,P=0.000）,the expression of Caspase-1 protein was positively correlated with mPAP.Conclusion By promoting the inflammation of pulmonary artery,Caspase-1 involves in the process of pulmonary arterial hypertension.Tea polyphenols could regulate the level of Caspase-1 in blood plasma and the expressions of Caspase-1,which may be linked wit

关 键 词：肺高压 茶多酚 半胱氨酸天冬氨酸蛋白酶-1 

分 类 号：Q95-33[生物学—动物学] R544.16[医药卫生—心血管疾病]