Metal sulfide quantum dots-aggregated PAMAM dendrimer for cadmium ion-selective electrode-based immunoassay of alpha-fetoprotein  

作　　者：Qunfang Li Jing Jin Fangming Lou Dianping Tang 

机构地区：[1]School of Chemistry and Environmental Engineering, Hubei University for Nationalities [2]Key Laboratory for Analytical Science of Food Safety and Biology of Ministry of Education, Fujian Province, State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fuzhou University

出　　处：《Science China Chemistry》2018年第6期750-756,共7页中国科学（化学英文版）

基　　金：supported by the National Natural Science Foundation of China (21675029, 21665009, 21475025);the Key Joint Project for Health Education of Fujian Province (WKJ2016-2-15);the Program for Changjiang Scholars and Innovative Research Team in University (IRT15R11)

摘　　要：An ion-selective electrode(ISE)-based immunoassay has been innovatively designed for the sensitive detection of liver cancer biomarker(alpha-fetoprotein,AFP),using metal sulfide quantum dot(QD)-based nano labels.Cd S QDs-aggregated PAMAM dendrimer(QD-DE)was first synthesized and functionalized with polyclonal rabbit anti-human AFP antibodies.Thereafter,a sandwich immunoreaction was implemented on monoclonal mouse anti-human AFP antibody-coated microplate by using antibody-functionalized QD-DE as the secondary antibody.Accompanying the immunocomplex,subsequent potentiometric detection of cadmium ion dissolved from the QD-DE under acidic condition was conducted on a portable cadmium ion-selective electrode(Cd-ISE).Results revealed that the electrode potential of the Cd-ISE increased with the increment of AFP concentration from 0.1 to 100 ng m L^(-1)at a detection limit(LOD)of 68 pg m L^(-1).The relative standard deviations(RSD)were below9.09%and 10.54%for the intra-and inter-assay,respectively.Additionally,six human serum specimens were determined on CdISE-based immunosensor by using commercial human AFP ELISA kit as the reference,and gave good relationship between two methods.Importantly,Cd-ISE-based immunoassay offers the promise for simple and cost-effective screening of disease-related biomarkers.An ion-selective electrode（ISE）-based immunoassay has been innovatively designed for the sensitive detection of liver cancer biomarker（alpha-fetoprotein,AFP）,using metal sulfide quantum dot（QD）-based nano labels.Cd S QDs-aggregated PAMAM dendrimer（QD-DE）was first synthesized and functionalized with polyclonal rabbit anti-human AFP antibodies.Thereafter,a sandwich immunoreaction was implemented on monoclonal mouse anti-human AFP antibody-coated microplate by using antibody-functionalized QD-DE as the secondary antibody.Accompanying the immunocomplex,subsequent potentiometric detection of cadmium ion dissolved from the QD-DE under acidic condition was conducted on a portable cadmium ion-selective electrode（Cd-ISE）.Results revealed that the electrode potential of the Cd-ISE increased with the increment of AFP concentration from 0.1 to 100 ng m L-1at a detection limit（LOD）of 68 pg m L-1.The relative standard deviations（RSD）were below9.09%and 10.54%for the intra-and inter-assay,respectively.Additionally,six human serum specimens were determined on CdISE-based immunosensor by using commercial human AFP ELISA kit as the reference,and gave good relationship between two methods.Importantly,Cd-ISE-based immunoassay offers the promise for simple and cost-effective screening of disease-related biomarkers.

关 键 词：potentiometric immunoassay metal sulfide quantum dot PAMAM dendrimer ALPHA-FETOPROTEIN ion-selective electrode cancer biomarker 

分 类 号：O657.1[理学—分析化学]