Novel Role of ER Stress and Mitochondria Stress in Serum-deprivation Induced Apoptosis of Rat Mesenchymal Stem Cells  

作　　者：Tong QIU Yan-yan HE Xiao ZHANG Xiao-lin MA 

机构地区：[1]State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Biomedical Material and Engineering Center, Wuhan University of Technology, Wuhan 430070, China [2]School of Materials Science and Engineering, Wuhan University of Technology, Wuhan 430070, China [3]Biomedical Materials and Engineering Research Center of Hubei Province, Wuhan University of Technology, Wuhan 430070, China [4]International School of Materials Science and Engineering, Wuhan University of Techonology, Wuhan 430070, China [5]Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients, Hubei University of Education, Wuhan 430205, China

出　　处：《Current Medical Science》2018年第2期229-235,共7页当代医学科学（英文）

基　　金：This study was supported by grants from the National Natural Science Foundation of China （No. NSC31300791） and the Opening Project of Hubei Key Laboratory of Purification and Application of Plant Anti-cancer Active Ingredients （No. HLPAI 2014006）.

摘　　要：The poor survival of mesenchymal stem cells （MSCs） compromises the efficacy of stem cell therapy. Growth factor deprivation is one of the important factors that have challenged the survival of donor MSCs in cell therapy. In this study, the aim was to evaluate the effect of serum deprivation on the cell death of MSCs and to investigate the underlying mechanisms. Apoptosis of MSCs was evaluated with Hoechst 33342/PI staining. Signaling pathways involved in serumdeprivation induced apoptosis were analyzed using Western blotting. The results revealed that serum deprivation induced apoptosis in MSCs within 72 h of treatment. Serum deprivation was shown to lead to protein expression alterations in Bax, Bcl-2, casepase-3, casepase-8, GRP78, and CHOP during experiments. The data suggested that the mitochondria death pathway, the extrinsic apoptotic pathway and the endoplastic reticulum（ER） stress pathway were all involved in MSCs apoptosis. The increase in expression of CHOP and the simultaneous decrease in Bcl- 2 expression suggest a synergistic effect in apoptosis induction in both the mitochondrion and the ER.The poor survival of mesenchymal stem cells （MSCs） compromises the efficacy of stem cell therapy. Growth factor deprivation is one of the important factors that have challenged the survival of donor MSCs in cell therapy. In this study, the aim was to evaluate the effect of serum deprivation on the cell death of MSCs and to investigate the underlying mechanisms. Apoptosis of MSCs was evaluated with Hoechst 33342/PI staining. Signaling pathways involved in serumdeprivation induced apoptosis were analyzed using Western blotting. The results revealed that serum deprivation induced apoptosis in MSCs within 72 h of treatment. Serum deprivation was shown to lead to protein expression alterations in Bax, Bcl-2, casepase-3, casepase-8, GRP78, and CHOP during experiments. The data suggested that the mitochondria death pathway, the extrinsic apoptotic pathway and the endoplastic reticulum（ER） stress pathway were all involved in MSCs apoptosis. The increase in expression of CHOP and the simultaneous decrease in Bcl- 2 expression suggest a synergistic effect in apoptosis induction in both the mitochondrion and the ER.

关 键 词：mesenchymal stem cells serum deprivation mitochondria pathway endoplasmicreticulum stress synergistic effects 

分 类 号：R[医药卫生]