双波长高效液相色谱法同时测定祛白颗粒中二苯乙烯苷、阿魏酸及丹皮酚的含量  

Simultaneous Determination of Stilbene Glycosides,Ferulic Acid and Paeonol in Qubai Granules by a Dualwavelength HPLC Method

作　　者：杨爱霞[1] 易凯 林世和[1] Yang Aixia;Yi Kai;Lin Shihe(Department of Pharmacy, Wuhan No.1 Hospital, Wuhan 430022, China)

机构地区：[1]武汉市第一医院药学部,武汉430022

出　　处：《中国药师》2018年第6期1093-1095,共3页China Pharmacist

基　　金：武汉市卫计委医学科研项目(编号:WZ13D21)

摘　　要：目的:建立双波长高效液相色谱切换法同时测定祛白颗粒中定量指标二苯乙烯苷、阿魏酸及丹皮酚含量的方法。方法:采用双波长高效液相色谱法,色谱柱:ODS(150 mm×4.6 mm,5μm);流动相:甲醇:0.1%磷酸为流动相进行梯度洗脱,流速:1.0 ml·min^(-1);柱温:35℃,进样体积:10μl,检测波长:丹皮酚为274 nm、阿魏酸和二苯乙烯苷为320 nm。结果:3种有效成分的线性范围分别为1.56~156.00μg·ml^(-1)(r_(二苯乙烯苷)=0.999 8)、1.00~100.0μg·ml^(-1)(r_(阿魏酸)=0.999 8),1.61~161.00μg·ml^(-1)(r_(丹皮酚)=0.999 7);平均加样回收率100.33%~100.76%,RSD均小于2%(n=6)。结论:该含量测定方法简便易行、稳定可靠、专属性强,可用于祛白颗粒的质量控制。Objective： To establish an HPLC determination method for the quantitative indicators in Qubai granules（ stilbene glycosides,ferulic acid and paeonol）. Methods： A dual-wavelength HPLC assay was used with the following conditions： a chromatography column ODS（ 150 mm×4. 6 mm,5 μm） was used,the mobile phase was methanol： 0. 1% phosphoric acid with gradient elution,the column temperature was 35℃,and the injection volume was 10 μl. Results： The linear ranges of the three active constituents were1. 56-156. 00 μg·ml-1（ rstilbene glycoside= 0. 999 8） 、1. 00 100. 00 μg·ml-1（ rferulic acid= 0. 999 8）,1. 61 161. 00 μg·ml-1（ rpaeonol=0. 999 7）,respectively,and the average recoveries of the three constituents were between 100. 33% and 100. 76% with the RSDSless than 2%（ n = 6）. Conclusion： The method is simple,stable and reliable,which can be used for the quality control of Qubai granules.

关 键 词：祛白颗粒 含量测定 二苯乙烯苷 阿魏酸 丹皮酚 双波长高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]