HPLC-DAD法测定迷迭香茎和叶中11种抗氧化活性成分  

Simultaneous determination of main anti-oxidant constituents in Rosmarinus officinalis by HPLC-DAD

作　　者：许艺凡[1] 刘普[1] 刘佩佩[1] 邱赛西 高晓丹 许肖锋 常盛 邓瑞雪[1] XU Yi-fan;LIU Pu;LIU Pei-pei;QIU Sai-xi;GAO Xiao-dan;XU Xiao-feng;CHANG Sheng;DENG Rui-xue(School of Chemical Engineering ＆ Pharmacy, Henan University of Science and Technology, Luoyang 471023, China)

机构地区：[1]河南科技大学化工与制药学院,河南洛阳471023

出　　处：《中草药》2018年第9期2153-2157,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(U1404831;U1604192);河南科技大学大学生训练计划项目资助(2017125);国家级大学生创新训练计划项目(201610464033)

摘　　要：目的建立HPLC-DAD同时测定迷迭香叶和茎中11种抗氧化活性成分含量的方法。方法采用Zorbax SB-Aq C18色谱柱(250 mm×4.6 mm,5μm);以甲醇(A)-磷酸二氢钾缓冲盐(B)溶液为流动相,梯度洗脱,体积流量1.0 mL/min,柱温30℃,绿原酸、咖啡酸、迷迭香酸、木犀草素、芹菜素和芫花素的检测波长为328 nm,丹皮酚、迷迭香酚、橙皮素、鼠尾草酚和鼠尾草酸的检测波长为284 nm,进样量10μL。结果绿原酸、咖啡酸、迷迭香酸、丹皮酚、迷迭香酚、橙皮素、木犀草素、芹菜素、鼠尾草酚、芫花素和鼠尾草酸分别在2.02~64.64μmol/mL,r=0.999 97;1.74~55.68μmol/mL,r=0.999 97;2.76~88.32μmol/mL,r=0.999 97;0.24~7.68μmol/mL,r=0.996 63;0.46~14.72μmol/mL,r=0.999 97;0.22~7.04μmol/mL,r=0.998 64;0.72~23.04μmol/mL,r=0.999 37;3.74~119.68μmol/mL,r=0.999 80;5.1~163.28μmol/mL,r=0.996 18;0.4~12.8μmol/mL,r=0.996 35;5.22~167.04μmol/mL,r=0.996 98内有良好的线性关系;平均加样回收率为98.1%~100.5%,RSD为0.67%~2.14%。结论该方法准确,实用性好,可用于迷迭香叶和茎中抗氧化活性成分的定性与定量分析。Objective To establish an HPLC-DAD method for simultaneous determination of main anti-oxidant constituents in leaves and stem of Rosmarinus officinalis. Methods The separation was performed on an Agilent Zorbax SB-Aq C18 column（250 mm × 4.6 mm,5 μm）, using methanol（A） and potassium dihydrogen phosphate solution（B）（p H 3.0） as the mobile phase at the flow rate of 1.0 mL/min for a gradient elution. The detection wavelength was set at 328 nm for the detection of chlorogenic acid, caffeic acid, rosmarinic acid, luteolin, apigenin, and genkwanin, and 284 nm for the detection of paeonol, rosmanol, hesperetin, carnosol, and carnosic acid. Column temperature was set at 30 ℃, and the volume of sample injection was 10 μL. Results The linear range was 2.02—64.64 μm/mL for chlorogenic acid, 1.74—55.68 μmol/mL for caffeic acid, 2.76—88.32 μmol/mL for rosmarinic acid, 0.24—7.68 μmol/mL for paeonol, 0.46—14.72 μmol/mL for rosmanol, 0.22—7.04 μmol/mL for hesperetin, 0.72—23.04 μmol/mL for luteolin, 3.74—119.68 μmol/mL for apigenin, 5.1—163.28 μmol/mL for carnosol, 0.4—12.8 μmol/mL for genkwanin, and 5.22—167.04 μmol/mL for carnosic acid. The average recoveries of the 11 constituents were from 98.1%—100.5%, and the RSD values were 0.67%—2.14%. Conclusion The method is simple and accurate,which can be used for quality and quantity analysis of the main anti-oxidant constituents in leaves and stem of R. officinalis.

关 键 词：迷迭香 高效液相色谱法 抗氧化成分 绿原酸 咖啡酸 迷迭香酸 木犀草素 芹菜素 芫花素 丹皮酚 迷迭香酚 橙皮素 鼠尾草酚 鼠尾草酸 

分 类 号：S788.1[农业科学—林学]