不同经穴组合针刺对失眠大鼠松果体褪黑素含量的影响  

Manual Acupuncture Stimulation of Paired Acupoints Can Relieve Sleep Disorder Possibly by Upregulating Pineal Melatonin Protein and Its Receptor mRNA Levels in the Suprachiasmatic Nucleus in Insomnia Rats

作　　者：郑雪娜[1] 吴雪芬[1] 郭鑫[1] 谢莉娜 谢志强 魏歆然[1] 刘丽[1] 陈小丽[1] 岳增辉[1] ZHENG Xue-na;WU Xue fen;GUO Xin;XIE Li na;XIE Zhi qiang;WEI Xin-ran;LIU Li;CHEN Xiao-li;YUE Zeng-hui(College of Acupuncture-moxihustion and Massage, Hunan University of Traditional Chinese Medicine ,Changsha 410208, China)

机构地区：[1]湖南中医药大学针灸推拿学院,长沙410208

出　　处：《针刺研究》2018年第6期360-364,共5页Acupuncture Research

基　　金：国家重点基础研究发展"973"计划项目(No.2014CB 543102);2017年湖南省研究生创新项目(No.CX 2017B454)

摘　　要：目的:观察针刺不同经脉穴位对失眠大鼠松果体褪黑素(MT)含量及视交叉上核(SCN)内褪黑素受体1(MT_1)、褪黑素受体2(MT_2)mRNA表达的影响,探讨固定局部穴位配伍不同经脉穴位之间作用的差异。方法:雄性SD大鼠随机分为空白组、模型组、百会+神门组、百会+三阴交组和百会+非经非穴组,每组12只。采用腹腔注射对氯苯丙氨酸混悬液建立失眠模型。治疗组每日针刺治疗1次,连续治疗7d。运用免疫组织化学法检测大鼠松果体MT的表达量,运用实时荧光定量PCR法检测大鼠SCN内MT_1、MT_2 mRNA的表达量。结果:与空白组比较,模型组大鼠松果体MT灰度值升高,SCN内MT_1、MT_2mRNA表达量降低(P<0.05,P<0.01)。与模型组比较,百会+神门组、百会+三阴交组松果体MT灰度值明显降低(P<0.05),百会+三阴交组、百会+神门组SCN内MT_1、MT_2mRNA表达量均明显升高(P<0.01)。各治疗组组间比较,百会+神门组MT_1mRNA表达量明显高于百会+非经非穴组(P<0.01),MT_2mRNA表达量明显高于百会+三阴交组及百会+非经非穴组(P<0.01)。结论:针刺治疗可以提高失眠大鼠松果体MT含量及SCN内MT_1、MT_2mRNA表达量,固定局部穴位配伍循经选穴可以提高腧穴配伍效应。Objective To observe the effect of manual acupuncture stimulation of paired ＂Baihui＂ （GV 20） ＋＂Shenmen＂ （HT 7）, GV 20 ＋ ＂Sanyinjiao＂ （SP 6）, and GV 20 ＋ non-acupoint on expression of melatonine （MT） and suprachiasmatic melatonin receptor 1 （MT1） and melatonin receptor 2 （MT2） mRNAs in insomnia rats, so as to explore their action difference and the underlying mechanism in improving insomnia. Methods Male SD rats were randomly divided into normal control （n = 12）, model （n=8）, GV20＋HT7（n=12）, GV20＋SP6（n=11）, andGV20＋non-acupeint （n=10） groups. The insomnia model was established by intraperitoneal injection of Para-chlorophenylalanine suspension （50 mg/mL, 50 rag/100 g）, once daily for 2 days. The abovementioned acupoints GV 20, bilateral HT 7, SP 6 and non-acupoints （the midpoint between the elbow-tip and armpit on the medial side of the upper-arm） were punctured with filiferm needles and manipulated by rotating the needle for about 1 min which was repeated once again every 10 min during 30 minutes＇ needle-retaining. The treatment was conducted once daily for 7 days. The expression levels of MT immunoactivity in the conarium tissue, and MT1 and MT2 mRNAs of the suprachiasmatic nucleus （SCN） region were detected using immunohistochemistry and fluorescence quantitative real time-POR, respectively. Results After modeling, the expression levels of pineal MT （an increase of gray value means a decrease of immunoactivity）, SCN MT1 and MT2 mRNAs were notably down-regulated in the model group relevant to the normal control group （P〈0.05, P〈0.01 ）. Following the treatment, the down-regulated expression levels of MT protein, and MT1 and MT2 mRNAs were obviously reversed in the GV 20 ＋ HT 7, GV 20 ＋ SP 6 groups relevant to the model group （P〈0.05, P〈0.01）. The therapeutic effect of GV 20＋HT 7 was su-perior to that of GV 20 ＋ non-acupoint in up-regulating the expression of MT1 mRNA （P〈0.01）, and markedly superior

关 键 词：失眠 针刺 腧穴配伍 褪黑素 褪素受体 视交叉上核 

分 类 号：R245.2[医药卫生—针灸推拿学]