香椿组培增殖和生根培养基筛选  

Screening of Proliferation and Rooting Medium for Tissue Culture of Toona sinensis

作　　者：高鷃铭 肖祥希[1] 王志洁[1] 张海燕[3] 何文广[1] 高楠[1] 肖华山[2] GAO Yanming;XIAO Xiangxi;WANG Zhijie;ZHANG Haiyan;HE Wenguang;GAO Nan;XIAO Huashan(Fujian Academy of Forestry ,Fuzhou 350012 ,Fujian, China;College of Life Science, Fujian Normal University, Fuzhou 350117, Fujian , China;Forestry Science and Technology Extension Center of Shaowu City, Shaowu 354000, Fujian, China)

机构地区：[1]福建省林业科学研究院,福建福州350012 [2]福建师范大学生命科学学院,福建福州350117 [3]福建省邵武市林业科技推广中心,福建邵武354000

出　　处：《福建林业科技》2018年第2期64-67,90,共5页Journal of Fujian Forestry Science and Technology

基　　金：福建省属公益类科研院所基本科研专项(2017R1011-1);福建省第四期林木种苗科技攻关项目(闽林科[2013]1号);福州市科技计划项目(榕科[2014]103号)

摘　　要：为研究香椿组培快繁技术,对香椿组织培养的培养基配方进行筛选。在MS基本培养基中添加不同浓度的6-BA、NAA、GA3的正交试验结果表明,影响香椿芽增殖的主要因素是6-BA,且对芽增殖的影响达显著水平(P<0.05),最佳质量浓度为1.0 mg·L^(-1);对芽增殖影响次要因素是NAA,最佳质量浓度为0.1 mg·L^(-1);GA3对芽增殖的影响最小,但对幼茎节间伸长生长有促进作用;芽增殖最佳培养基为MS+6-BA 1.0 mg·L^(-1)+NAA 0.1 mg·L^(-1)+GA30.1 mg·L^(-1),芽增殖倍数可达3.6倍。以MS、1/2 MS为基本培养基添加不同浓度的NAA、IBA进行的生根培养试验结果表明:MS+NAA 0.2mg·L^(-1)+IBA 0.2 mg·L^(-1)培养基生根效果最好,生根率可达25%。腐殖土作为香椿组培苗移栽基质,组培苗移栽成活率可达95%,苗木长势好。In order to study the tissue culture and rapid propagation of Toona sinensis,the medium formula for tissue culture of Toona sinensis was studied and screened.By adding different concentrations of 6-BA,NAA and GA3 in MS medium,the orthogonal experiment and variance analysis showed that the main factor affecting the proliferation of Toona sinensis was 6-BA,which had a significant effect on the bud proliferation（P 〈0.05）,and the best concentration was 1.0 mg·L^（-1）;the secondary factor affecting bud proliferation was NAA,and the optimal concentration was 0.1 mg·L^（-1）;GA3 had the least effect on bud proliferation,but GA3 had a promoting effect on the internode elongation growth of young stems.The optimal bud culture medium combination was MS ＋ 6-BA 1.0 mg·L^（-1）＋ NAA 0.1 mg·L^（-1）＋ GA30.1 mg·L^（-1）,and the multiplication ratio of shoots reached 3.6 times.MS and 1/2 MS medium supplemented with different concentrations of NAA and IBA to carry out rooting culture test,the results showed that the MS ＋ NAA0.2 mg·L^（-1）＋ IBA 0.2 mg·L^（-1） formula combination medium had the best rooting effect,and the rooting rate was 25%.Taking humus soil as tissue culture seedlings transplanting toothache,the survival rate of the tissue culture seedlings transplanted was high up to 95%,and the seedlings grew well.

关 键 词：香椿 组织培养 芽增殖 生根率 培养基 

分 类 号：S723.123.6[农业科学—林木遗传育种]