多菌灵降解菌的生物学特性及降解能力研究  

Biological Characteristic and Biodegradation Capacity of Ochrobactrum tritici WNP-3 for Carbendazim

作　　者：陈锐[1] 孙晓宇[1] 邓媛[1] 路鹏鹏[1] 赵玲侠[1] 瞿佳[1] 沈卫荣[1] CHEN Rui;SUN Xiao-yu;DENG Yuan;LU Peng-peng;ZHAO Ling-xia;QU Jia;SHEN Wei-rong(Shaanxi Province Institute of Microbiology, Xi＇ an 710043)

机构地区：[1]陕西省微生物研究所,西安710043

出　　处：《生物技术通报》2018年第5期187-194,共8页Biotechnology Bulletin

基　　金：陕西省科学院青年人才培养项目(2013K-25);陕西省农业科技创新与公关项目(2015NY088);省科技厅科技统筹创新计划(2016FWPT-10)

摘　　要：农药污染已成为影响人身健康及环境的主要原因。在污染土壤中引入降解特定农药的微生物可有效降低该农药的浓度,并且该方法绿色环保、无二次污染。为降低棚室土壤中的残留农药多菌灵,通过唯一碳源平板筛选法,从长期使用多菌灵的棚室土壤中分离筛选出一株分解利用多菌灵的微生物菌株WNP-3,该菌株可在含200 mg/L的多菌灵LB培养基中正常生长。经形态学、理化、Biolog及16S rDNA鉴定,确定该菌为小麦苍白杆菌(Ochrobactrum tritici)。该菌表现出宽泛的生长范围:在pH4-9,盐含量1%-3%条件下正常生长,对多种农药具有耐受性。经紫外分光光度法及HPLC验证WNP-3在无机盐培养基、28℃、180r/min摇瓶培养120 h的条件下,对100 mg/L多菌灵的降解率为61%。Pesticide pollution becomes major cause to environment safety and human health. Introducing microorganisms of degrading specific pesticide into polluted soil may efficiently reduce the concentration of this pesticide;moreover,this is a green technology andcauses no secondary pollution. In order to reduce the residual carbendazim in the soil of a greenhouse,a strain WNP-3 decomposing andutilizing carbendazim was isolated via sole carbon source plate screening method from the greenhouse soil where carbendazim has been usedfor a long time. This strain normally grew in LB medium with carbendazim concentration of 200 mg/L. According to the results of morphology,physiological and biochemical property,Biolog identification,and 16 S rDNA sequence analysis,WNP-3 was identified as Ochrobactrum tritici. The WNP-3 had broad growth range,i.e.,growing well at pH 4-9 in culture media containing 1%-3% Na Cl,and it was resistant toseveral pesticides. Confirmed by UV spectrophotometry and HPLC,the carbendazim at concentration of 100 mg/L degraded 61% in mineral medium in 180 r/min flask for 120 h at 28℃.

关 键 词：细菌 多菌灵 小麦苍白杆菌 生物降解 

分 类 号：X172[环境科学与工程—环境科学]