E3泛素连接酶PARK2对人脑胶质瘤细胞侵袭与迁移能力的影响  

E3 Ligase PARK2 Affects Glioblastoma Multiforme(GBM) Cell Invasion and Migration

作　　者：吴珏[1] 裘佳寅 许璐[1] 李锦[1] 陈宁 潘亭亭 田亚平[1] WU Jue1,QIU Jiayin2,XU Lu1, LI Jin1, CHEN Ning1, PAN Tingting1,TIAN Yaping1(1 Laboratory of Translational Medicine, Chinese PLA General Hospital, Beijing 100853, China;2 School of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou Zhejiang 310053, Chin)

机构地区：[1]中国人民解放军总医院转化医学实验室,北京100853 [2]浙江中医药大学药学院,浙江杭州310053

出　　处：《中国卫生标准管理》2018年第10期116-119,共4页China Health Standard Management

基　　金：国家自然科学基金项目(81503116)

摘　　要：目的探讨E3泛素连接酶PARK2影响脑胶质瘤细胞侵袭和迁移作用。方法采用免疫印迹法检测PARK2蛋白在脑胶质瘤细胞系中的表达情况;选用逆转录病毒载体p BABE-puro vector构建PARK2-WT(野生型)的稳定过表达细胞系;采用细胞划痕实验观察过表达PARK2后U251细胞迁移能力的变化;Transwell侵袭实验观察细胞侵袭能力的变化;Real-time PCR检测MMP1、MMP12和MMP13基因表达水平。结果筛选脑胶质瘤细胞中PARK2蛋白表达水平,U251和T98G蛋白表达水平较低,并构建U251和T98G细胞上PARK2过表达株;脑胶质瘤细胞中PARK2过表达后,细胞迁移能力显著降低(P<0.01);U251和T98G细胞中PARK2过表达组相较于转染空载体的对照组穿过Matrigel基质胶及滤膜的细胞数明显减少,差异有统计学意义(P<0.01)。结论过表达PARK2可以抑制脑胶质瘤细胞的侵袭和迁移能力。Objective To study the relationship between the protein level of E3 Ligase PARK2 and glioma progression and the effect of PARK2 on the invasive and migratory ability of glioma cells. Methods The protein expression level of PARK2 was detected by Western blotin glioma cell lines. Next the effect of PARK2 overexpression on the migratory and invasive ability of glioma cells were examined by wound healing assay and matrigel precoated transwell chambers, respectively. Results The protein level of PARK2 was higher expression in human gliomas cell of U138 and A172 than U251, T98 G and U373. The number of cells acmssing the wound edge decreased in the group of PARK2 overexpression in U251（P 〈 0.01）. In the matrigel invasion assay, cell number invading through matrigel dramatically decreased in the group of PARK2 overexpression in U251 and T98 G（P 〈 0.01）. Conclusion The expression level of PARK2 in human gliomas was higher in U138 and A172. Overexpression of PARK2 in U251 and T98 G inhibited glioma cell invasion and migration.

关 键 词：神经胶质瘤 E3泛素连接酶 PARK2 细胞迁移 侵袭 

分 类 号：R739.41[医药卫生—肿瘤]