声脉冲辐射力成像下甲状腺癌的临床评估以及OPN、OPNmRNA表达的相关性分析  

Clinical Evaluation of Thyroid Cancer in Sound Pulsed Radiographic Imaging and Correlation Analysis of OPN and OPN mRNA Expression

作　　者：冯岚[1] 景香香[2] 陶阳[1] 邢秀红[1] 袁汝亮[1] 方丽妮 郑福雄 FENG Lan;JING Xiang-xiang;TAO Yang;XING Xiu-hong;YUAN Ru-liang;FANG Li-ni;ZHENG Fu-xiong(Functional Department,Hainan Provincial Third People＇s Hospital,Sanya,Hainan,572000;Department of Ultrasound,Hainan Provincial people＇s Hospital,Haihou Hainan,570311)

机构地区：[1]海南省第三人民医院功能科,海南三亚57200 [2]海南省人民医院超声科,海南海口570311

出　　处：《海南医学院学报》2018年第9期971-974,978,共5页Journal of Hainan Medical University

基　　金：三亚市医疗卫生科技创新项目(2015YW33)~~

摘　　要：目的:探讨甲状腺癌在声脉冲辐射力成像的临床评估及骨桥蛋白表达的相关性分析。方法:选取海南省甲状腺癌(thyroid carcinoma,TC)患者60例共71个病灶,所有病例均采用TI-RADS分级标准分级,并经手术后病理证实确定诊断。采用免疫组织化学S-P法检测OPN水平表达,荧光定量聚合酶链反应(FQ-PCR)测定OPN mRNA含量,并对声脉冲辐射力成像与OPN表达的进行相关性分析。结果:60例TC患者中原发性甲状腺癌56例(93.33%),继发性甲状腺癌4例(6.67%);其中存在砂粒样结节钙化型37例,占61.67%;采用TI-RADS分级,3级5例(8.33%),4级37例(61.67%),5级18例(30.00%);根据病理学特征分型:甲状腺乳头性癌32例(53.33%),弥漫硬化性癌19例(31.67%),滤泡性癌9例(15.00%)。影像表现中37例钙化型甲状腺癌OPNmRNA的含量明显高于非钙化型甲状腺癌(P<0.05),且OPN表达水平升高与砂粒样钙化分布情况成正比趋势(P<0.05)。结论:甲状腺癌钙化型微粒体的分布与OPN的表达水平呈正相关趋势,提示OPN表达增强可能与砂粒体的形成、结节的硬度增加、恶性度升高相关联。声脉冲辐射力成像技术对甲状腺癌的早期诊断及预测甲状腺癌的预后具有重要意义。Objective： To investigate the clinical assessment of thyroid carcinoma in acoustic pulse radiation imaging and the correlation analysis of osteopontin expression. Methods： Totally 71 lesions of 60 patients with thyroid carcinoma （TC） in Hainan were selected. All patients were graded by TI-RADS grading and confirmed by pathology after operation. The expression of OPN was detected by immunohistochemical S-P method. The expression of OPN mRNA was detected by fluorescence quantitative polymerase chain reaction （FQ-PCR）. The correlation between OPN expression and acoustic pulse radiation imaging was analyzed. Results： ① 60 patients （93.33%） with primary thyroid cancer and four （6.67%） patients with secondary thyroid cancer were found in 60 patients with TC. Thirty-seven patients （61.67% According to the pathological features, 32 cases （53.33%） had papillary thyroid carcinoma, diffuse sclerosis 19 cases of cancer （31.67%）, follicular carcinoma in 9 cases （15.00%）. ②The expression of OPN mRNA in 37 cases of calcified thyroid carcinoma was significantly higher than that in non-calcified thyroid carcinoma （ P 〈0.05）, and the increase of OPN expression was in direct proportion to the distribution of gritty calcification （ P 〈0.05）. Conclution： The distribution of calcified microsomes in thyroid cancer was positively correlated with the expression of OPN, suggesting that enhanced OPN expression may be related to the formation of sand bodies, the increased hardness of nodules and the increased malignancy. Acoustic pulse radiation imaging technology for early diagnosis of thyroid cancer and prognosis of thyroid cancer was of great significance.

关 键 词：甲状腺癌 声脉冲辐射力成像 骨桥蛋白 

分 类 号：R736.1[医药卫生—肿瘤]