异甘草素对人胶质瘤细胞系U251增殖、凋亡的影响及相关机制研究  

The Effect of Isoliquiritigenin on the Proliferation and Apoptosis of Human Glioma Cell Line U251 and Its Related Mechanism

作　　者：邓云秋 DENG Yunqiu(Department of Neurosurgery,Tianjin Jinghai District Hospital 30160)

机构地区：[1]天津市静海区医院神经外科,301600

出　　处：《医学理论与实践》2018年第9期1260-1261,1270,共3页The Journal of Medical Theory and Practice

摘　　要：目的:探析异甘草素对脑胶质瘤U251细胞增殖、凋亡的影响,并总结其相关机制。方法:采用浓度不等的异甘草素处理脑胶质瘤U251细胞样本,同时对10、20、40、60、80μmol/L各浓度设5个复孔,均处理24h、48h、72h;采取流式细胞术对细胞凋亡变化检测,由四甲基偶氮唑蓝(MTT)对细胞增殖能力检测,核糖体RNA(mRNA)、叉头框蛋白M1(FoxM1)表达水平则采取逆转录-聚合酶链反应(RT-PCR)法检测。结果:脑胶质瘤U251细胞经浓度不同的异甘草素处理后,细胞增殖均受到程度不一的抑制表现,在同作用时间,但不同浓度之间差异具有统计学意义(P<0.05);反之,在相同浓度,但作用时间不同细胞增殖情况的差异同样具有统计学意义(P<0.05);在对细胞凋亡影响方面,随着异甘草素浓度不同作用细胞24h后,细胞凋亡率随着异甘草素浓度增加出现正比增加趋势(P<0.05);在FoxM1、mRNA相对表达水平中,不同浓度间异甘草素处理细胞48h后差异具有统计学意义(P<0.05)。结论:异甘草素可理想抑制脑胶质瘤U251细胞增殖,并有效诱导其凋亡作用机制,这可能与下FoxM1基因表达水平正相关。Objective：To investigate the effect of iso glycyrrhizin on the proliferation and apoptosis of glioma U251 cells,and to summarize the related mechanisms.Methods：Using different concentrations of glycyrrhizin treatment samples of glioma U251 cells,and in 10,20,40,60,80 different concentration ofμmol/L with 5 holes,24 h,48 h,72 hwere taken on the change of apoptosis;flow cytometry,four by methyl thiazolyl tetrazolium（MTT）on cell proliferation detection,ribosomal RNA（mRNA）,forkhead box protein M1（FoxM1）expression by reverse transcriptase polymerase chain reaction（RT-PCR）assay.Results：Glioma U251 cell proliferation was inhibited the level of performance,at the same time,but the difference between different concentrations were statistically significant（P〈0.05）;on the other hand,in the same concentration,but the effect of different time of cell proliferation was also statistically significant（P〈0.05）;the effect on cell apoptosis,with different concentration of isoliquiritigenin cells after 24 h,apoptosis rate with isoliquiritin concentration increased proportional increase（P〈0.05）;the level of FoxM1 and mRNA expression in different concentration of isoliquiritigenin treated cells 48 hwas statistically significant difference（P〈0.05）.Conclusion：Iso glycyrrhizin has an inhibitory effect on the proliferation and apoptosis of glioma U251 cells,which may be positively correlated with the expression level of FoxM1 gene.

关 键 词：异甘草素 脑胶质瘤 细胞增殖 细胞凋亡 机制 

分 类 号：R73-3[医药卫生—肿瘤]