经寰枕间隙侧方穿刺移植人脐血单个核细胞对难治性神经系统疾病的疗效分析  

Safety of transplantation of umbilical cord blood mononuclear cells by the lateral atlantooccipital space puncture for treatment of refractory neurological diseases

作　　者：于海燕[1] 宫殿荣[1] 袁晓玲[1] 刘力峰[1] 赵敏[1] 王敏[1] 芦倩 Yu Haiyan, Gong Dianrong, Yuan Xiaoling, Liu Lifeng, Zhao Min, Wang Min, Lu Qian(Department of Neurology, Liaocheng People＇s Hospital, Liaocheng 252000, Chin)

机构地区：[1]聊城市人民医院神经内科,252000

出　　处：《中华细胞与干细胞杂志（电子版）》2018年第2期115-119,共5页Chinese Journal of Cell and Stem Cell（Electronic Edition）

摘　　要：目的探讨寰枕间隙侧方穿刺术移植人脐血单个核细胞治疗难治性神经系统疾病的可行性和安全性。方法应用寰枕间隙侧方穿刺术对230例患者进行450次人脐血单个核细胞治疗,观察治疗中及治疗后有无不良反应和并发症,对比治疗前后血液分析、血沉、生化全项、出凝血机制和肿瘤标记物数值,同时观察患者治疗前后病情转归,采用配对比较t检验进行统计学分析。结果所有患者治疗后均无头痛、感染、皮疹、血肿形成等不良反应和其它移植并发症出现。32例(7.1﹪)治疗后出现一过性血压升高,8例(1.8﹪)出现一过性发热,10例(2.2﹪)穿刺时诉穿刺处深部软组织胀痛,拔针后疼痛消失。患者白细胞计数治疗前(7.9±1.1)×10~9个/L和治疗后3个月(8.0±1.3)×10~9个/L相比差异无统计学意义(t=0.891,P=0.374),谷丙转氨酶治疗前(31.9±5.8)U/L和治疗后3个月(32.4±6.2)U/L相比差异无统计学意义(t=0.893,P=0.372),球蛋白治疗前(22.1±1.7)g/L和治疗后3个月(21.8±1.8)g/L相比差异无统计学意义(t=0.838,P=0.066),AFP治疗前(9.9±1.6)μg/L和治疗后3个月(10.1±1.7)μg/L相比差异无统计学意义(t=1.299,P=0.195),患者血液学指标(血常规+血沉、生化全项、全身肿瘤标记物、病毒筛查、出凝血机制)在治疗前后无统计学差异。183例患者治疗有效,有效率79.6﹪。结论寰枕间隙侧方穿刺术移植人脐血单个核细胞治疗难治性神经系统疾病是安全可行并可能有效的。Objective To investigate the safety and effectiveness of umbilical cord blood mononuclear cells transplantation therapy by the lateral atlanto-occipital space puncture. Methods 230 patients have received umbilical cord blood mononuclear cell therapy for 450 times by the lateral atlanto occipital space puncture. The adverse reactions and complications during treatment and after treatment were observed, the hematological parameters were compared before and after treatment, and the patient＇s condition was observed before and after treatment. Statistical analysis was carried out by two samples compared with t test. Result All the patients had no headache, infection, hematoma formation, adverse reactions and other complications after treatment. Transient blood pressure increased after treatment in 32 patients（ 7.1﹪）, and transient fever occurred in 8（1.8 ﹪）. 10（2.2 ﹪） patients experienced deep soft tissue pain at the time of the puncture. There was no statistical difference（t = 0.891, P = 0.374） between the leukocyte count before（7.9±1.1）and 3 months after treatment（8.0±1.3）. There was no statistical difference（t = 0.893, P = 0.372）between the glutamic pyruvic transaminase（μ/L） before（31.9±5.8） and 3 months after treatment（32.4±6.2）. There was no statistical difference（t = 1.838, P = 0.066） between the globulin before（22.1±1.7） and 3 months after treatment（21.8±1.8）. There was no statistical difference（t = 1.299, P = 0.195） between the AFP count before（9.9±1.6）and 3 months after treatment（10.1±1.7）.The hematological indexes（blood routine ＋ ESR, biochemical markers, tumor markers, virus screening, coagulation and coagulation mechanisms） and vital signs of all patients were not statistically different before and after treatment. 183 patients were treated effectively（the effective rate was 79.6﹪）.Conclusion Umbilical cord blood mononuclear cells transplantation therapy by lateral atlantooccipital space puncture is safe and appear

关 键 词：寰枕间隙侧方穿刺术 人脐血单个核细胞 移植 安全性 

分 类 号：R741[医药卫生—神经病学与精神病学]