ω-3多不饱和脂肪酸对脂多糖所致脑损伤新生大鼠海马组织中氧化应激产物和细胞凋亡的影响  

Effect of ω-3 polyunsaturated fatty acid on oxidative stress production and apoptosis in hippocampus of neonatal rats with brain injury induced by lipopolysaccharide

作　　者：石计朋[1] 栗延伟[2] 郭丽娟[3] 张利利[4] 何晓敬 王卫卫 郭洪旭[1] 高俊[1] 郝洁[5] 黄倩[6] 唐成和[1] SHI Ji-peng;LI Yan-wei;GUO Li-juan;ZHANG Li-li;HE Xiao-jing;WANG Wei-wei;GUO Hong- xun;GAO Jun;HAO Jie;HUAGN Qian;TANG Cheng-he(Department of Neonatology, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Provinee, China;Department of Neurology, the Third Affiliated Hospital of Xinxiang Medical University,Xinxiang 453003, Henan Provinee,China;Department of Uhrasonography, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Provinee, China;Department of Gastroenterology, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Province, China;Department of Infection Disease, the First Affiliated Hospital of Xinxiang Medical University, Weihui 453100, Henan Provinee, China;Department of Paediatrics, the First Affiliated Hospital of Xinxiang Medical University , Weihui 453100, Henan Provinee , China)

机构地区：[1]新乡医学院第一附属医院新生儿科,河南卫辉453100 [2]新乡医学院第三附属医院神经内科,河南新乡453003 [3]新乡医学院第一附属医院超声科,河南卫辉453100 [4]新乡医学院第一附属医院消化内科,河南卫辉453100 [5]新乡医学院第一附属医院感染疾病科,河南卫辉453100 [6]新乡医学院第一附属医院儿内科,河南卫辉453100

出　　处：《新乡医学院学报》2018年第6期455-458,463,共5页Journal of Xinxiang Medical University

基　　金：河南省医学科技攻关计划项目(编号:201602148);河南省神经修复重点实验室开放课题(编号:HNSJXF-2016-017)

摘　　要：目的探讨ω-3多不饱和脂肪酸(PUFA)对脂多糖(LPS)所致脑损伤新生大鼠海马组织中氧化应激产物和细胞凋亡的影响。方法将48只3日龄新生Sprague Dawley大鼠随机分为对照组、LPS组、ω-3 PUFA组和ω-6 PUFA组,每组12只;LPS组、ω-3 PUFA组和ω-6 PUFA组新生大鼠腹腔注射0.6 mg·kg-1LPS后分别立即腹腔注射等容积生理盐水、ω-3 PUFA和ω-6 PUFA;对照组大鼠腹腔注射等容积生理盐水;24 h后快速断头取海马组织,检测各组新生大鼠海马组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、氧化型GSH(GSSG)水平,并计算GSSG/GSH比值;原位末端转移酶标记法检测各组新生大鼠海马组织细胞凋亡指数(AI)。结果与对照组比较,LPS组、ω-6 PUFA组和ω-3 PUFA组新生大鼠海马组织中SOD和GSH水平显著降低(P<0.05),MDA、GSSG水平及GSSG/GSH比值显著升高(P<0.05)。与LPS组比较,ω-6 PUFA组新生大鼠海马组织中SOD和GSH水平显著降低(P<0.05),MDA、GSSG水平及GSSG/GSH比值显著升高(P<0.05);ω-3 PUFA组新生大鼠海马组织中SOD和GSH水平显著升高(P<0.05),MDA、GSSG水平及GSSG/GSH比值显著降低(P<0.05)。与ω-6 PUFA组比较,ω-3 PUFA组新生大鼠海马组织中SOD和GSH水平显著升高(P<0.05),MDA、GSSG水平及GSSG/GSH比值显著降低(P<0.05)。LPS组、ω-6 PUFA组和ω-3 PUFA组新生大鼠海马组织AI显著高于对照组(P<0.05);ω-6 PUFA组新生大鼠海马组织AI显著高于LPS组(P<0.05);ω-3 PUFA组新生大鼠海马组织AI显著低于ω-6 PUFA组和LPS组(P<0.05)。结论ω-3 PUFA能够减轻LPS所致脑损伤新生大鼠海马组织中的氧化应激反应,减少海马组织细胞凋亡,对LPS所致的脑损伤具有保护作用。Objective To investigate the effects of ω-3 polyunsaturated fatty acid（PUFA） on oxidative stress production and apoptosis in hippocampus of neonatal rats with brain injury induced by lipopolysaccharide（LPS）. Methods Fortyeight neonatal Sprague Dawley rats（3 days） were randomly divided into control group,LPS group,ω-3 PUFA group and ω-6 PUFA group,with 12 rats in each group. The rats in the LPS group,ω-3 PUFA group and ω-6 PUFA group were given0. 6 mg·kg-1 LPS via intraperitoneal injection,then equal volume of saline,ω-3 PUFA and ω-6 PUFA was immediately given via intraperitoneal injection respectively; while the rats in the control group were all given equal volume of saline. The rats in each group were sacrificed at 24 hours after intraperitoneal injection with saline or fat emulsions to obtain the hippocampus. The levels of superoxide dismutase（SOD） and malondialdehyde（MDA）,reduced glutathion（GSH）,oxidized glutathione（GSSG）were detected and GSSG/GSH was calculated. The apoptotic index was measured by terminal deoxynucleotidyl transferase-mediated d UTP nick end labeling. Results Compared with the control group,the levels of SOD and GSH in hippocampus of rats in the LPS group,ω-6 PUFA group and ω-3 PUFA group were significantly decreased（P〈0. 05）,and the levels of MDA,GSSG and the ratio of GSSG/GSH were significantly increased（P〈0. 05）. Compared with the LPS group,the levels of SOD and GSH in hippocampus of rats in ω-6 PUFA group were significantly decreased（P〈0. 05）,the levels of MDA,GSSG and the ratio of GSSG/GSH were significantly increased（P〈0. 05）; the levels of SOD and GSH in hippocampus of rats in ω-3 PUFA group were significantly increased（P〈0. 05）,the levels of MDA,GSSG and the ratio of GSSG/GSH were significantly decreased（P〈0. 05）. Compared with the ω-6 PUFA group,the levels of SOD and GSH in hippocampus of rats in ω-3 PUFA group were significantly decreased（P〈0. 05）,the levels of MDA,GSSG and the ratio of GSSG/GSH wer

关 键 词：多不饱和脂肪酸 脑损伤 脂多糖 超氧化物歧化酶 丙二醛 谷胱甘肽 细胞凋亡 

分 类 号：R965[医药卫生—药理学]