大孔/介孔多级孔SiO_2的制备及其在固定化脂肪酶中的应用  被引量:8

Preparation of Hierarchical Macroporous/Mesoporous Silica and Its Application in Lipase Immobilization

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作  者:李笑迎[1] 白文静[1] 陶凯[1] 梁云霄[1] LI Xiaoying;BAI Wenjing;TAO Kai;LIANG Yunxiao(State Key Laboratory Base of Novel Functional Materials and Preparation Science, Faculty of Materials Science andChemical Engineering, Ningbo University, Ningbo 315211)

机构地区:[1]宁波大学材料科学与化学工程学院新型功能材料及其制备科学国家重点实验室培育基地,宁波315211

出  处:《材料导报》2018年第10期1695-1700,1715,共7页Materials Reports

基  金:浙江省公益项目(2014C31130);宁波大学王宽诚幸福基金(XKL072)

摘  要:以具有三维骨架结构的环氧树脂大孔聚合物为模板,制备具有毫米级尺寸的大孔/介孔多级孔SiO_2。应用SEM、MIP、FTIR和N_2吸附-脱附法对材料孔道结构和表面性质进行表征。采用吸附法固定褶皱假丝酵母脂肪酶(CRL),研究CRL初始浓度、pH值及固定化时间对脂肪酶固定化的影响,对比研究了游离脂肪酶和固定化脂肪酶的酶学性质。结果表明,大孔/介孔SiO_2具有三维连续贯通的大孔孔道,孔壁由连续的SiO_2纳米薄膜构筑而成且表面存在丰富的介孔,比表面积为75.1m^2/g,孔隙率为92.3%;在CRL浓度为0.6mg/mL、pH值为8.0、固定化时间为10h时,固定化酶酶活达到4 825U/g。与游离脂肪酶相比,固定化脂肪酶的pH稳定性、热稳定性和储存稳定性明显提高,连续使用8次后的酶活为初始酶活的68%。利用环氧树脂大孔聚合物模板制备的大孔/介孔多级孔SiO_2在固定化酶方面具有良好的应用前景。Millimeter-sized hierarchical macroporous/mesoporous silica was prepared by using an epoxy resin macroporous polymer with three-dimensional(3D)skeletal structure as template.Pore structures and surface properties of the as-prepared materials were characterized by SEM,MIP,FTIR and N2adsorption-desorption.Candida rugosa lipase(CRL)was immobilized on the macroporous/mesoporous silica by adsorption method.The effects of the initial CRL concentration,pH and immobilizing time on lipase immobilization were investigated,and the properties of free and immobilized lipase were studied.The results demonstrated that the macroporous/mesoporous silica had 3Dcontinuous pass-through macroporous structure;its pore wall was constructed by continuous silica nano-film and had abundant mesopores.The specific surface area and porosity are 75.1m^2/g and 92.3%,respectively.The activity of immobilized lipase reaches 4 825U/g under conditions of initial CRL concentration 0.6mg/mL,pH of 8.0and immobilizing time of 10h.Compared with free CRL,the pH,thermal and storage stabilities of immobilized lipase were improved significantly.The immobilized lipase retained 68%residual activity after 8consecutive operations.Therefore,this hierarchical macroporous/mesoporous silica was a good potential candidate for enzyme immobilization.

关 键 词:二氧化硅 大孔/介孔 多级孔 固定化酶 褶皱假丝酵母脂肪酶 

分 类 号:Q814.2[生物学—生物工程]

 

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