复方苦参注射液对荷DMS153裸鼠移植瘤的抑瘤作用及STAT3、MMP9表达的影响  

Effects of compound Kushen injection on antitumor activity and expression of STAT3 and MMP9 in nude mice modeled with DMS153 cells

作　　者：黄明华[1] 肖明生[1] 陈传军 樊拖迎[1] 吴军 Huang Minghua;Xiao Mingsheng;Chen Chuanjun;Fan Tuoying;Wu Jun(Chinese People＇s Liberation Army NO.94 Hospital,Jiangxi Nanchang 330000,China;Nanchang Royo Biotech Co.,Ltd,Jiangxi Nanchang 330029,China.)

机构地区：[1]中国人民解放军第九四医院,江西南昌330000 [2]南昌乐悠生物科技有限公司,江西南昌330029

出　　处：《现代肿瘤医学》2018年第11期1675-1680,共6页Journal of Modern Oncology

摘　　要：目的:观察复方苦参注射液(compound Kushen injection,CKI)对荷DMS153小细胞肺癌裸鼠移植瘤的抑瘤作用,并初步探讨其作用机制与STAT3、MMP9表达的关系。方法:建立荷DMS153裸鼠移植瘤模型。实验分为对照组、CKI低中高剂量干预组、顺铂干预组,共5组,每组10只裸鼠。CKI低中高干预组每天予以不同剂量CKI干预[1.0、2.0和4.0 ml/(kg·d)],顺铂(DDP)干预组予以顺铂干预(第1、3、5天给药4.8mg/kg),每天观察移植瘤生长情况,并测量肿瘤体积,各实验组裸鼠在第29天处死取材。q PCR和Western blotting法检测肿瘤中STAT3和MMP9的mRNA和蛋白表达水平。结果:相比于对照组,CKI不同剂量干预均能不同程度抑制肿瘤的生长(P<0.05),其中高剂量组抑制作用最为明显;与对照组相比,DDP干预亦能够显著抑制移植瘤生长(P<0.05),与CKI高剂量干预组相比差异具有统计学意义(P<0.05)。与对照组相比,CKI不同剂量干预均能不同程度抑制移植瘤中STAT3和MMP9的mRNA转录和蛋白表达水平(P<0.05),其中高剂量组抑制最为显著;CKI不同剂量干预组STAT3和MMP9的mRNA转录呈显著正相关(Pearson相关系数为0.912>0,P=0.044<0.05);CKI不同剂量干预组STAT3和MMP9的蛋白表达水平均呈显著正相关(Pearson相关系数为0.990>0,P=0.005<0.05);与对照组相比,DDP干预亦能够显著抑制移植瘤组织中STAT3和MMP9的mRNA转录和蛋白表达(P<0.05),与CKI高剂量干预组相比差异亦具有统计学意义(P<0.05)。结论:CKI能够有效抑制荷DMS153小细胞肺癌裸鼠移植瘤的生长,且高剂量组抑瘤作用较优;CKI抗小细胞肺癌的作用机制可能与其调控STAT3和MMP9的基因和蛋白表达有关。Objective：To observe the antitumor effects of compound Kushen injection（ CKI） on nude mice modeled with DMS153 cells,and to explore the mechanism of its relationship with STAT3 and MMP9 expression.Methods：To establish nude mice model of small cell lung cancer derived from DMS153 cell line.The experiment included5 groups：Control group,CKI low,middle and high dose intervention group and cisplatin intervention group,and 10 mice in each group.Group CKI received different doses of CKI intervention every day.Group DDP received DDP intervention.The growth of transplanted tumor was observed every day,and the volume of tumor was measured.The nude mice in each experimental group were sacrificed at twenty-ninth days.q PCR and Western blotting were used to detect the expression of STAT3 and MMP9 genes and proteins in tumors.Results：Compared with control,different doses of CKI intervention could inhibit tumor growth（ P〈0.05） in different degrees,in which the high dose group had the most obvious inhibitory effect.Compared with control,DDP intervention could significantly inhibit the growth of transplanted tumor（ P〈0.05）,which was statistically significant compared with CKI high dose intervention group（ P〈0.05）.Compared with control,different doses of CKI intervention could inhibit the expression of STAT3 and MMP9 mRNA and protein（ P〈0.05） in transplanted tumor,and the high dose group was the most significant.There were significant positive correlations between mRNA and protein expression levels of STAT3 and MMP9 in different doses of CKI（ correlation coefficients were 0.912 and 0.990,P = 0.044 and 0.005）.Compared with control,DDP intervention could significantly inhibit the mRNA transcription and protein expression of STAT3 and MMP9（ P〈0.05） in transplanted tumor tissues,and the difference was statistically significant（ P〈0.05） compared with CKI high dose intervention group.Conclusion：CKI can inhibit the growth of DMS153 small cell lung cancer xenografts in nude mice,and th

关 键 词：复方苦参注射液 小细胞肺癌 STAT3 MMP9 

分 类 号：R734.2[医药卫生—肿瘤]