游泳运动减轻急性肺损伤的影响及机制研究  

Swimming exercise attenuates acute lung injury and related mechanisms

作　　者：陈建国 袁继芳 姜同仁 钱峰[2] CHEN Jianguo;YUAN Jifang;JIANG Tongren;QIAN Feng

机构地区：[1]安徽财经大学体育教学部,安徽蚌埠233000 [2]上海交通大学医学部,上海200025

出　　处：《淮南师范学院学报》2018年第2期87-91,共5页Journal of Huainan Normal University

基　　金：国家自然科学基金"蛋白激酶p38alpha MAPK调控巨噬细胞极化在特发性肺纤维化中的作用机制研究与新型药物靶标"(81573438)

摘　　要：目的:探讨游泳运动对小鼠急性肺损伤的影响,分析运动改善急性肺损伤的可能作用机制。方法:构建LPS诱导的急性肺损伤模型,通过H&E染色、肺湿干比、MPO活性、荧光定量PCR及免疫印迹等方法判断炎症的严重程度。结果:1)H&E染色结果显示,运动干预后,LPS诱导的炎症症状减轻。2)运动干预后,肺湿干比具有显著性差异(P<0.05),肺灌洗液(BALF)总蛋白浓度显著减低(P<0.01),MPO活性显著降低(P<0.001)。3)LPS运动训练组CD11b+Gr-1+细胞比率明显低于LPS对照组。4)RT-PCR检测促炎因子(包括IL-1β、TNF-α和IL-6),结果显示LPS运动训练组促炎因子m RNA水平低于LPS对照组,且具有显著性差异(P<0.05)。5)运动训练后,显著降低P38 MAPK磷酸化水平(P<0.01)。结论:游泳运动能降低LPS诱导的炎性细胞浸润和促炎因子的表达,同时可以减轻肺组织通透性及肺组织损伤从而改善LPS引起的急性肺损伤。Objective： The purpose of the study was to determine the role of swimming in acute lung injury, and explore the possible mechanisms. Methods： To build the model of acute lung injury induced by LPS, the severity of inflammation was determined by HE staining, lung wet dry ratio, MPO activity,fluorescence quantitative PCR and immunoblotting. Results： 1） The results of HE staining showed that LPS induced inflammatory symptoms were reduced after exercise. 2） After exercise, there was a significant difference in lung wet dry ratio（P〈0.05）, total protein concentration in lung lavage fluid（BALF） was significantly decreased（P〈0.01）, and MPO activity was significantly decreased（P〈0.001）.3） The ratio of CD11 b＋Gr-1＋ cells in the LPS exercise group was significantly lower than that of the LPS control group 4）Compared to groups A and B, the m RNA expression of IL-1β,TNF-α and IL-6 in group C were increased significantly, while the IL-1β,TNF-α and IL-6 m RNA expression in group D was decreased significantly（P〈0.05）. 5） After exercise, the phosphorylation level of P38 MAPK was significantly reduced（P〈0.01）.Conclusions： 6-week regular swimming exercise can relieve LPS-induced ALI by inhibit the infiltration of inflammatory cells, the expression of pro-inflammatory cytokines, the permeability and injury of lung tissue.

关 键 词：游泳运动 LPS 急性肺损伤 P38 MAPK 

分 类 号：G806[文化科学—运动人体科学]