环境中抗药菌株的分离、鉴定及其抑菌活性研究  

Isolation,Identification and Antimicrobial Traits of Antibiotic Resistant Strains in Environment

作　　者：阎春兰[1] 央青卓玛 韦英美 王涛 雷世庭 王磊 YAN Chun-lan;YANG-QING Zhuo-ma;WEI Ying-mei;WANG Tao;LEI Shi-ting;WANG Lei(College of Life Sciences,South-Central University for Nationalities,Wuhan 430074, Chin)

机构地区：[1]中南民族大学生命科学学院,武汉430074

出　　处：《湖北农业科学》2018年第10期60-65,共6页Hubei Agricultural Sciences

基　　金：中央高校基本科研业务费专项(CZW15105);中南民族大学大学生科研基金项目(XCX16101)

摘　　要：采用革兰氏染色技术、生理生化试验和16S rDNA序列分析,对分离筛选自土壤中的抗药菌株进行鉴定;测定菌株的生长曲线了解菌株的生长情况;耐药性和抑菌性试验检测菌株的特性。结果表明,从土壤中分离得到11种抗药菌株,经鉴定为假单胞菌属(Pseudomonas)、贪噬菌属(Variovorax)、溶杆菌属(Lysobacte)和短芽孢杆菌属(Brevibacillus)等。其中,菌株XC-10、XN-4、XN-5和XN-6等分别对金黄色葡萄球菌(Staphylococcus aureus)、枯草芽孢杆菌(Bacillus subtilis)和藤黄微球菌(Micrococcus luteus)等细菌的生长有不同程度的抑制作用;11种菌株分别对终浓度为10μg/mL壮观霉素和20μg/mL氨苄青霉素等具有显著的耐药性。By using Gram stain, physiological property and phylogenetic trees based on 16S rDNA,the different antibiotic resistant strains which were screened and isolated from soil were identified. Then the growth chart of strains were measured,and the drug resistance and the antimicrobial characteriatics were detected. The results showed that 11 antibiotic resistant strains were isolated and identified from soil. According to morphological characteristics and the phylogenetical analysis of 16S rDNA,these strains were determined to belong to Pseudomonas, Variovorax, Lysobacte and Brevibacillus. And the strains of XC-10,XN-4,XN-5 and XN-6 had different inhibitory effects on the growth of bacteria such as Staphylococcus aureus,Bacillus subtilis,and Micrococcus luteus. Screened had obvious inhibiting effect on six common indicator strains. The 11 strains all showed significant drug-resistance to the final concentration of 10 μg/mL spectinomycin and 20 μg/mL ampicillin.

关 键 词：抗药菌株 分离鉴定 16S RDNA 抑菌特性 

分 类 号：Q93-331[生物学—微生物学]