儿童复发性腮腺炎患者与正常儿童腮腺液微生物群落多样性的差异  

Difference of the parotid microflora Alpha diversity of microflora between juvenile recurrent parotitis(JRP) and the normal children

作　　者：李莹[1] 祁森荣[2] 华琳[3] 尚佳健[1] Li Ying;Qi Senrong;Hua Lin;Shang Jiajian(Department of Pediatric, Beijing Stomatological Hospital,Capital Medical University,Beijing 100050, China;Department of Radiology, Beijing Stomatological Hospital,Capital Medical University,Beijing 100050, China;Department of Mathematics and Bioinformatics,School of Biomedical Engineering,Capital Medical University,Beijing 100069, China)

机构地区：[1]首都医科大学附属北京口腔医院儿童口腔科,北京100050 [2]首都医科大学附属北京口腔医院放射科,北京100050 [3]首都医科大学生物医学工程学院数学与生物信息教研室,北京100069

出　　处：《首都医科大学学报》2018年第3期453-457,共5页Journal of Capital Medical University

摘　　要：目的通过检测儿童复发性腮腺炎患者与正常儿童腮腺液的微生物群落分布的变化情况,分析二者之间微生物群落多样性的差异。方法通过使用Illumina Hiseq 2500 PE250高通量测序平台对34例儿童复发性腮腺炎患者和16例正常儿童腮腺液标本中微生物群落的16SrRNA进行多样性分析,并对两组样本数据进行统计比较。结果与正常儿童腮腺液对比,儿童复发性腮腺炎患者腮腺液Alpha多样性指标中样品文库的覆盖率(goods_coverage)增高(P<0.05),Chao1、菌群丰富度指数(abundance coverage estimator,ACE)、Shannon指数降低(P<0.05),Simpson指数差异无统计学意义。结论儿童复发性腮腺炎患者腮腺液微生态环境中微生物群落的多样性与正常儿童腮腺液差异有统计学意义。Objective To investigate the difference in the richness and diversity of the parotid microflora between juvenile recurrent parotitis（ JRP） and the normal children. Methods The parotid saliva specimens were obtained from JRP and normal children（ 34 specimens from JRP,16 specimens from normal children） to analyze the diversity of the 16 SrRNA gene sequence of of the microorganism using Illumina Hiseq 2500 PE250 high throughput sequencing platform. Results Compared with normal children,JRP had higher goods_coverage（ P〈0. 05）,lower Chao1,abundance coverage estimator（ ACE）,Shannon（ P〈0. 05） and lower Simpson（ P〉0. 05）.Conclusion Significant changes in the richness and diversity of parotid microflora between JRP and the normal children.

关 键 词：儿童复发性腮腺炎 口腔微生物群落 多样性 丰富度 腮腺液 

分 类 号：R788[医药卫生—口腔医学]