云南省双柏县一犬伤多人事件调查及狂犬病病毒进化分析  

Investigation of the dog-biting events and molecular evolution characteristics of rabies virus strains in Shuangbai county of Yunnan province

作　　者：郭兴祥 杨卫红[2] 冯云[2] 章域震[2] 周芬 李映金 张海林[2] Guo Xingxiang;Yang Weihong;Feng Yun;Zhang Yuzhen;Zhou Fen;Li Yingjin;Zhang Hailin(Chuxiong Prefecture Center for Disease Control and Prevention, Chuxiong 675000, Chin;Yannan Institute of Endemic Diseases Control and Prevention/Yunnan Provincial Key Laboratory for Zoonosis Control and Prevention, Dali 671000, Chin;Shuangbai County Center for Disease Control and Prevention, Shuangbai 675100, Chin)

机构地区：[1]楚雄州疾病预防控制中心,675000 [2]云南省地方病防治所云南省自然疫源性疾病防控技术重点实验室,大理671000 [3]双柏县疾病预防控制中心,675100

出　　处：《中华实验和临床病毒学杂志》2018年第3期237-241,共5页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的阐明云南省双柏县一犬伤多人事件流行病学特点及狂犬病病毒（Rabies virus，RV）流行株分子进化特征。方法对一犬伤多人事件和犴犬病病例进行流行病学调杏，采集肇事犬及狂犬病死广病人脑组织标本，用RT—PCR扩增RV核蛋白基闲序列，用相关生物信息学软件进行同源性和系统进化分析。结果2011—2017年双柏县共发生12起一犬伤多人事件，犬伤暴露35例。其中11起一犬伤多人事件的32例犬伤暴露人员均进行伤口处理和犴犬病疫苗全程接种，至今未发病；而仅有一起一犬伤3人事件（2014年5月）因漏报而未做规范处置，导致1人患犴犬病。经RT-PCR和序列测定，获得2012—2014年双柏县6株RV核蛋白基因序列（犬源5株和人源1株）。进化和同源性分析表明，双柏县流行株与相邻楚雄市和禄丰县、普洱市景东县、大理州祥云县和四川省RV China—I进化群流行株进化关系较近；双柏县流行株间及其与相邻楚雄市和禄丰县、大理州祥云县和四川省近期RVChina—I流行株核苷酸和氨基酸同源性均为99．6％～100％，但与2006-2007年楚雄州、昭通和曲靖市RVChina—I流行株核苷酸和氨基酸同源性分别为97．1％～99．3％和99．1％～99．6％。结论开展一犬伤多人事件监测和规范处置能有效防控人狂犬病，双柏县RV流行株均属China—I进化群并与相邻州、市、县和四川省流行株具有较近亲缘关系和较高同源性。Objective To illustrate the epidemieal characteristics of the dog-biting events and molecular evolution of rabies virus （RV） strains prevalent in Shuangbai county of Yunnan province, China. Methods Epidemical investigation on the dog-biting events and human cases were conducted and the brain tissues of the biting dogs and human cases were sampled post-mortem. Nucleoprotein （N） genes of the RVs were sequenced. Homology and phylogenetic analysis were performed using the relevant bioinformatics software. Results A total of 12 dog-biting events took place between 2011-2017 in Shuangbai county and 35 persons were bitten. Of the 12 events, 11 were investigated in time and 32 bitten persons received proper wound management and a full post-exposure vaccination course. Rabies has not developed in these wounded cases until now. However, due to failure to receive medical intervention and post-exposure treatment in time, 1 of 3 bitten persons in a single event died of rabies. RV N genes from 5 dogs and I person were sequenced. Phylogenetie tree showed that RV strains prevalent in Shuangbai county were closely related with the ones found in neighboring counties/cities such as Chuxiong, Lufeng, Jingdong and Xiangyun. All these strains were related to the ones denoted as clade China-I and prevalent in Sichuan province. Homology analysis showed 99.6%-100% homology in nucleotide and amino acid among the 6 RVs prevalent in Shuangbai county and those prevalent in Chuxiong, Lufeng, Xiangyun and clade China-I of Sichuan provinee. Compared with the China-I strains prevalent in Chuxiong, Zhaotong and Qujing prefectures between 2006-2007, the homology of nucleotide and amino acid were 97. 1% -99.3% and 99. 1% -99.6% , respectively. Conclusions Surveillance on the dog-biting events can prevent rabies in humans efteetively. RV strains prevalent in Shuangbai county belong to clade China-I and have a close relationship with those of neighboring prefectures, cities, counties and the ones prevalent in Sichuan province.

关 键 词：狂犬病 暴露后预防处置 狂犬病病毒 核蛋白基因 进化分析 

分 类 号：R512.99[医药卫生—内科学]