珠海市吸毒人群丙型肝炎病毒分子流行病学研究  

Molecular epidemiological study on hepatitis C virus among drug users in Zhuhai, China

作　　者：李红霞[1] 魏泉德[1] 周毅[1] Li Hongxia;Wei Quangde;Zhou Yi(Zhuhai Center for Disease Control and Prevention, Zhuhai 51900, China)

机构地区：[1]珠海市疾病预防控制中心,519000

出　　处：《中华实验和临床病毒学杂志》2018年第3期259-262,共4页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的了解珠海市吸毒人群丙型肝炎病毒分子流行病学情况。方法采用ELISA法检测血浆抗HCV、real-time RT-PCR法检测HCV RNA，对NS5B区扩增子直接测序，与GenBank比对确定HCV基因型和同源性，构建系统进化树并分析。结果435份吸毒者血浆样本中，242份抗HCV和HCV RNA均阴性，147份抗HCV和HCV RNA均阳性，46份HCV RNA或抗HCV抗体阳性，HCV感染率44.37%。78份HCV RNA阳性样本成功检出3个基因型和5个基因亚型，分别是6 a，60.26%；3b，15.38%；3 a，14.10%；1b，6.41%和1 a, 3.85%。与进化树中毒株同源的有我国深圳、佛山、广州、汕尾、中国香港HCV毒株和来自于丹麦、加拿大、美国、英国、印度、尼泊尔等国家的毒株。结论珠海市吸毒人群中流行的HCV与多个国家和地区的毒株同源。珠海吸毒人群优势HCV为6 a亚型，新检出1 a和3a亚型。Objective To investigate the molecular epidemiology of hepatitis C virus （HCV） among drug users in Zhuhai, China. Methods Anti-HCV and HCV RNA were deteeted by using ELISA and real-time RT-PCR respectively. HCV genotypes and subtypes were determined by direct sequencing of amplicons in partial NSSB regions and BLAST GenBank, followed by phylogenetic analysis. Results Of 435 blood plasma from drug users, 242 was negative for anti-HCV and HCV RNA, 147 were positive for anti-HCV and HCV RNA, 46 positive HCV RNA or anti-HCV. The infection rate of HCV was 44.37%. Three genotypes and five subtypes of HCV were detected in 78 samples of positive HCV RNA. Subtypes 6 a, 3b, 3 a, lb anti 1 a, were detected at frequencies of 60.26% , 15.38% , 14. 10%, 6.41% and 3.85%, respectively. Compared with strains from Shenzhen, Foshan, Guangzhou, Shanwei, Hong Kong, Denmark, Canada, USA, United Kingdom, India and Nepal, HCV among drug users in Zhuhai were homophylic. Conclusions There were homology among HCV in drug users from Zhuhai and strains from some regions and countries. Subtype 6 a was the most predominant. Subtype 1 a and 3 a were discovered in Zhuhai.

关 键 词：吸毒人群 丙型肝炎病毒 基因型 系统进化树 同源性 

分 类 号：R181.3[医药卫生—流行病学] R512.63[医药卫生—公共卫生与预防医学]