长链非编码RNAHOTAIR对舌鳞状细胞癌增殖及迁移侵袭能力的影响  

Effects of Long Non-coding RNA HOTAIR on Proliferation,Migration,and Invasion of Tongue Squamous Cell Carcinoma

作　　者：代梦莹[1] 张洋[1] 张照楠 宿伟鹏[1] 张宋安[1] 刘攀[1] 赵化荣[1] DAI Meng-ying;ZHANG Yang;ZHANG Zhao-nan;SU Wei-peng;ZHANG Song-an;LIU Pan;ZHAO Hua-rong(Tumor Centre, The First Affiliated Hospital, Xinjiang Medical University, Urumqi 830054, China)

机构地区：[1]新疆医科大学第一附属医院肿瘤中心,新疆乌鲁木齐830054

出　　处：《口腔医学研究》2018年第6期627-631,共5页Journal of Oral Science Research

基　　金：新疆维吾尔自治区自然科学基金(编号:2016D01C263)

摘　　要：目的:探讨长链非编码RNA HOTAIR对舌鳞状细胞癌细胞系增殖及迁移侵袭能力的影响。方法:采用脂质体介导小干扰RNA(small interfering RNA,siRNA)敲低HOTAIR的表达,将无意义序列设为对照,采用定量反转录聚合酶链反应(quantificational revere transcriotion-polymerase chain reaction,qRT-PCR)检测沉默效果。噻唑蓝(methyl thiazol tetrazolium,MTT)法检测各组细胞增殖能力的变化,划痕实验、Transwell实验检测各组细胞侵袭迁移能力的变化。结果:2株细胞系中HOTAIR成功敲低,差异具有统计学意义(P<0.001);在MTT实验中,与对照组比较,实验组细胞24、48、72、96h时吸光度(A)值均降低(P<0.05);Transwell实验中实验组穿膜细胞数明显少于对照组(P<0.001),划痕实验中实验组的迁移距离少于对照组(P<0.001)。结论:HOTAIR促进了舌鳞状细胞癌细胞Tca-8113、TSCCA的增殖、迁移与侵袭,参与了舌癌的发展过程。Objective：To investigate the effect of HOTAIR on proliferation,migration,and invasion of tongue squamous cell carcinoma cell line.Methods：The expression of HOTAIR was knocked down by liposome mediated siRNA,and the meaningless sequence was set as the control group.QRT-PCR was used to detect the silencing effect.MTT assay was used to detect the proliferation of each group.The changes of invasion and migration ability of each group was detected by scratch test and Transwell test.Results：HOTAIR was successfully knocked down in the 2 cell lines,and the difference was statistically significant（P〈0.001）.In the MTT assay,compared with the control group,the absorbance values after 24 h,48 h,72 h,and 96 hin the experimental group were decreased（P〈0.05）.In the Transwell test,the membrane cell number of the experimental group was significantly less than that of the control group（P〈0.001）.The migration distance of the experimental group in the scratch group was less than that of the control group（P〈0.001）.Conclusion：HOTAIR promotes the proliferation,migration,and invasion of tongue squamous cells carcinoma（Tca-8113,TSCCA）,which involved in the development of tongue cancer.

关 键 词：舌鳞状细胞癌 长链非编码RNA HOTAIR 增殖 迁移 侵袭 

分 类 号：R739.86[医药卫生—肿瘤]