超重小鼠应对牙周感染的血清炎症因子反应  

Serum Cytokine Response to Periodontal Infection in Overweight Mice

作　　者：余挺[1] 赵莉[2] 章锦才 轩东英 YU Ting;ZHAO Li;ZHANG Jin-cai;XUAN Dong-ying(Department of Periodontology, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510150, China;Department of Prosthodontics, Guanghua School of Stomatology, Sun Yatsen University and Guangdong Provincial Key Laboratory of Stomatology, Guangzhou 510055, China;Savaid Medical School, University of Chinese Academy of Sciences, Beijing 100049, China;Department of Periodontology, Hangzhou Dental Hospital, Savaid Medical School, University of Chinese Academy of Sciences, Hangzhou 310006, China.)

机构地区：[1]广州医科大学附属口腔医院·广州口腔疾病研究所·口腔医学重点实验室牙周科,广东广州510140 [2]中山大学光华口腔医学院·附属口腔医院,广东省口腔医学重点实验室口腔修复科,广东广州510055 [3]中国科学院大学·存济医学院,北京100049 [4]中国科学院大学存济医学院杭州口腔医院牙周科,浙江杭州310006

出　　处：《口腔医学研究》2018年第6期644-647,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81700985;81371151;81470750);广州市卫生和计划生育科技项目(编号:20181A011102)

摘　　要：目的:探索超重宿主应对牙周感染的系统性免疫反应。方法:将28只C57BL/6J小鼠分为高脂(HFD)和低脂饲料(LFD)组,HFD诱导超重模型,LFD作为正常体重对照。诱导8周时,两饲料组均再分为牙周炎(P)和健康(C)组(n=7),P组做牙周结扎,C组做对照。10d后处死,Micro-CT分析骨丧失线性分数,蛋白芯片筛检血清中20个炎症因子水平,单因素方差分析比较4组间差异。结果:HFD组的体重高于LFD组3.8g(15.5%)(P<0.01)。P组的骨丧失线性分数显著高于C组(P<0.01)。除HFDP组的IL-6水平较LFDC组显著上调外(P<0.05),其他炎症因子反应在HFD与LFD组间无显著差异,在HFD小鼠的P组与C组间亦无差异。结论:未发现系统性炎症介导超重与牙周炎的相互关系。Objective：To explore systemic immune response of overweight host to periodontal infection.Methods：Twenty-eight C57 BL/6 Jmice were divided into high-（HFD）or low-fat diet（LFD）groups to induce overweight or being as controls,respectively.After 8 weeks on diet,both two diet groups were subdivided into periodontitis（P）or periodontal-health controls（C）（n=7）,with the P group for periodontal ligation and the C group as controls.The mice were killed after 10 days＇ ligation.Linear bone loss fraction was analyzed by micro-CT.Twenty candidate serum cytokines were detected by protein arrays.Differences between the four groups were analyzed by one-way ANOVA.Results：The HFD group had 3.8 g or 15.5% higher body weight than that of the LFD group（P〈0.01）.The P group had significantly higher linear bone loss fraction compared with that of the C group（P〈0.01）.Except for IL-6,the level of which was significantly higher in the HFDP group relative to the LFDC group（P〈0.05）,no any other serum cytokines showed significantly different levels between HFD and LFD groups or between P and C groups within the HFD mice.Conclusion：No significant mediating role of systemic inflammation is found in correlation between overweight and periodontitis.

关 键 词：肥胖 超重 牙周炎 炎症 细胞因子 

分 类 号：R781.42[医药卫生—口腔医学]