CCK8和PGE2对CVB体外攻击人外周血pDC的调控  

Duplex effect of CCK8 and PGE2 in CVB-attacked human peripheral blood pDC

作　　者：贾苗苗 齐莉莉 李慧 高翔 刘文宣[3] 贾娴娴 JIA Miao-Miao;QI Li-Li;LI Hui;GAO Xiang;LIU Wen-Xuan;JIA Xian-Xian(Department of Pathogenic Biology, Institute of Basic Medical Science, Hebei Medical University, Shijiazhuang 050017, China)

机构地区：[1]河北医科大学基础医学院病原生物学教研室,石家庄050017 [2]石家庄市第三医院,石家庄050011 [3]河北医科大学公共卫生学院,石家庄050017

出　　处：《中国免疫学杂志》2018年第6期820-825,共6页Chinese Journal of Immunology

基　　金：河北省自然科学基金(H2015206378);河北省高等学校青年拔尖人才计划项目(BJ2014045);河北省高等学校高层次人才科学研究项目(GCC2014004)

摘　　要：目的:了解八肽胆囊收缩素(CCK8)是否参与柯萨奇病毒B(CVB)体外攻击人外周血浆细胞样树突状细胞(p DC)的调控。方法:RT-PCR和IF检测CCK受体的表达;FCM分析协同刺激分子的变化,用ELISA和RT-PCR检测IFN-α的分泌。结果:CVB体外攻击p DC,CCK1R和CCK2R的表达增加;CCK8可以抑制CD80、CD86和HLA-DR表达的增高,而PGE2可以进一步促进CD80、CD86和HLA-DR的表达;同时,CCK8抑制CVB攻击后IFN-α的分泌,而PGE2进一步促进IFN-α的分泌。结论:CCK8和PGE2可双向调控CVB体外攻击人外周血p DC的免疫应答。Objective：To investigate the immunomodulation of CCK8 on the Coxsackievirus B（CVB）-attacked human peripheral blood plasmacytoid dendritic cells（p DC）.Methods：Peripheral blood mononuclear cells of healthy volunteers were separated by Ficoll-Hypaque gradient density centrifugation.The p DC was separated and divided into five groups,which were the control group,CVB attacked group,the group of CCK8 treated after CVB attack,the group of PGE2 treated after CVB attack and the group of CCK8＋PGE2 treated after CVB attack.100-time TCID50 of CVB was applied for the attack on p DC.Real-time PCR and Immunofluorescence technique were employed to detect the expression of CCK1 R/CCK2 R mRNA and protein.Then,the expression levels of costimulatory molecules such as CD80,CD86,HLA-DR ligand,and the chemokine receptor CCR7 were evaluated by Flow Cytometry Analysis.The supernatants of p DCs were collected,and the content of IFN-α was determined by Enzyme-linked Immunosorbent Assay.Results：CCK1 R and CCK2 R were co-expressed in human peripheral blood p DC,and both were significantly upregulated after CVB attack in vitro.Expression of CD80,CD86,HLA-DR and IFN-α were decreased in the CVB ＋ CCK8 group compared with the CVB group,which suggested that CCK8 may reduce the CVB activation of p DC.Whereas expression of CD80,CD86,HLA-DR,CCR7 and IFN-α were increased in the CVB ＋ PGE2 group compared with the CVB group,which suggested that PGE2 may increase the CVB activation of p DC in vitro.Conclusion：CCK8 repressed the CVB-attacked p DC,while PGE2 activated the CVB-attacked p DC.

关 键 词：浆细胞样树突状细胞 柯萨奇病毒B CCK8 PGE2 

分 类 号：R392[医药卫生—免疫学]