姜黄素抑制CD44^+结肠癌细胞增殖的体外实验研究  

Proliferation inhibition effects of curcumin against CD44^+ colon cancer cells in vitro

作　　者：郗雪艳 付飞 樊旭 杜伯雨 XI Xue-Yan;FU Fei;FAN Xu;DU Bo-Yu(School of Basic Medical Sciences, Hubei University of Medicine, Shiyan 442000, Chin)

机构地区：[1]湖北医药学院基础医学院,十堰442000

出　　处：《中国免疫学杂志》2018年第6期850-853,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金应急管理项目(81650019);湖北省科技厅面上项目(2017CFB551;2017CFB462)

摘　　要：目的:探讨姜黄素能否抑制CD44^+结肠癌细胞的增殖。方法:MTT法检测姜黄素处理的结肠癌细胞增殖情况,Real-time PCR检测姜黄素处理的结肠癌细胞CD44 mRNA的表达水平,流式细胞术检测姜黄素处理的结肠癌细胞表面CD44的表达水平,磁珠分选方法分离得到结肠癌细胞中CD44^+细胞和CD44-细胞,MTT法观察姜黄素对两种细胞增殖情况的影响。结果:姜黄素可抑制结肠癌细胞HCT116和HT-29增殖,Real-time PCR和流式细胞术均证明姜黄素可导致结肠癌细胞CD44表达下调,磁珠分选方法获得CD44^+的结肠癌细胞并应用姜黄素处理后,结果提示姜黄素可抑制CD44^+细胞增殖。结论:姜黄素可通过抑制结肠癌肿瘤干细胞增殖而降低结肠癌复发和转移,有望成为结肠癌治疗的辅助性药物。Objective：To study confirm whether curcumin could inhibit the proliferation of CD44^＋colon cancer cells.In this study.Methods：MTT method was used to test the proliferation inhibition effects of curcumin against colon cancer cells.And Real-time PCR and Fluorescence-activated cell sorting（FACS） were used to determine CD44 expression level in colon cancer cells after treated with curcumin.Then we used Magnetic-activated cell sorting（MACS） method to separate CD44 positive and negative cells.And we compared the proliferation inhibition effects of curcumin against these two kinds of cells.Results：Curcumin could inhibit the proliferation of HCT116 and HT-29 colon cancer cell lines.The expression level of CD44 could also be down-regulated after the colon cancer cells were treated with curcumin.Conclusion：Curcumin could inhibit the proliferation of CD44^＋colon cancer cells.Thus it was suggested that crcumin might have the effect to inhibit the proliferation of colon cancer stem cells and might be used as a promising agents to prevent the relapse and metastasis of colon cancer.

关 键 词：姜黄素 结肠癌 肿瘤干细胞 CD44^+ 

分 类 号：R735.3[医药卫生—肿瘤]