丘脑前核电刺激对癫痫大鼠海马神经再生作用的影响  

Influences of anterior thalamic nucleus stimulation on neurogenesis in hippocampus of epileptic rats

作　　者：石林[1] 周加林[2] 朱冠宇[1] 陈颍川 Shi Lin;Zhou Jialin;Zhu Guanyu;Chert Yingchuan(Department of Neurosurgery , Beijing Tiantan Hospital, Capital Medical Universit;Beijing Neurosurgical Institute, Beifing 100050, China （ Shi L, Zhu G Y , Chen YC;Department of Medicament, Beijing No. 6 Hospital, Beiring 100007, China（ Zhou JL)

机构地区：[1]首都医科大学附属北京天坛医院神经外科、北京市神经外科研究所,北京100050 [2]北京市第六医院药剂科,北京100007

出　　处：《中华行为医学与脑科学杂志》2018年第6期481-486,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目（81701268）

摘　　要：目的探讨丘脑前核（anterior thalamic nucleus，ANT）电刺激对颞叶癫痫大鼠海马神经元再生作用的影响。方法将32只雄性SD大鼠随机分为正常对照组、正常电刺激组、模型假刺激组、模型电刺激组，每组8只。采用海马CA3区微量注射海人酸建立SD大鼠颞叶癫痫模型，进行慢性期连续48hANT电刺激，观察大鼠癫痫发作频率、发作类型的变化，通过Western blot及qRT．PCR分析大鼠海马组织中Ki-67等神经再生相关蛋白表达水平的变化。结果模型假刺激组和模型电刺激组动物癫痫发作总次数分别为[（5．9±2．2）次／周，（2．9±1．1）次／周；P〈0．05]，ANT电刺激使癫痫大鼠的癫痫发作频率降低了50．8％（p〈0．05）。Western blot蛋白分析结果显示：与模型假刺激组动物相比，模型电刺激组大鼠海马组织神经再生相关蛋白Ki-67的相对含量明显升高[（0．19±0．73），（0．44±0．15），P〈0．05）]。qRT-PCR实验结果显示：与模型假刺激组动物相比，模型电刺激组动物海马组织神经再生相关蛋白Ki-67的mRNA相对含量明显升高[（0．45±0．10），（0．15±0．06）；P〈0．05]。结论长时程ANT电刺激可以使癫痫大鼠海马组织神经再生作用增强，这可能是ANT电刺激治疗癫痫的重要机制。Objective To investigate the influences of anterior thalamic nucleus （ANT） stimulation on neurogenesis in hippocampus of epileptic rats. Methods Thirty-two male SD rats were randomly allocated to normal-control group （ n = 8 ）, control-stimulation group （ n = 8 ）, sham-stimulation group （ n = 8 ） and model-stimulation group（ n= 8）.Eqileptic SD rat model was established by using microinjection of kainic acid in hippocampal CA3 area,and administered 48 h continuous ANT stimulation in the chronic stage.Epileptic seizures were monitored and counted.The levels of Ki-67, a neurogenesis protein in hippocampus was determined by Western blot and quantitative real-time polymerase chain reaction （qRT-PCR）. Results The epilepsy seizure rate was （5.9±2.2） per week in the sham-stimulation group and （2.9±1.1） per week in model- stimulation group. Compared with sham-stimulated rats, ANT stimulation reduced seizures by 50.8% （P〈 0.05） .Western blot analysis revealed that the relative levels of Ki-67 in the hippocampus of model-stimulation group significantly higher than that of the sham-stimulation group （（ 0.44± 0.15 ） vs （0. 19± 0.73 ） , P〈 0.05） .qRT-PCR analysis showed that relative levels of Ki-67 mRNA in the hippoeampus of model-stimulation group were significantly higher than that of the sham-stimulation group（（0.45±0.10） vs （0. 15±0.06）, P〈0.05 ）. Conclusion Chronic ANT stimulation can promote neurogenesis in epileptic rats, which may be a principle mechanism of the beneficial effect of ANT stimulation on epilepsy.

关 键 词：丘脑前核 电刺激 神经再生 癫痫 

分 类 号：R742.1[医药卫生—神经病学与精神病学]