NOB1对宫颈癌细胞糖酵解及凋亡影响的实验研究  

Effects of NOB1 on apoptosis and glycolysis in human cervical cancer cells

作　　者：杨栋霞[1] 马桂霞[1] YANG Dongxia;MA Guixia(Department of Gynecology, the First People＇s Hospital of Zhengzhou, Zhengzhou 450004, He＇nan, Chin)

机构地区：[1]郑州市第一人民医院妇科,郑州450004

出　　处：《癌症进展》2018年第6期694-697,共4页Oncology Progress

摘　　要：目的探讨NOB1对宫颈癌细胞凋亡和糖酵解的影响。方法宫颈癌细胞He La转染NOB1 si RNA、si RNA control后命名为NOB1 si RNA组、si RNA control组,以未转染的细胞为对照组。RT-PCR和Western blot法检测转染效果,流式细胞术检测细胞凋亡情况,试剂盒检测己糖激酶Ⅱ(HK-Ⅱ)和乳酸脱氢酶(LDH)活性,Western blot法检测β-连环蛋白(β-catenin)和c-myc蛋白表达情况。结果 si RNA control组细胞中NOB1 m RNA和蛋白水平与对照组比较,差异均无统计学意义(P﹥0.05);NOB1 si RNA组细胞中NOB1 m RNA和蛋白水平均低于对照组,差异均有统计学意义(P﹤0.05)。NOB1 si RNA组细胞与对照组比较,凋亡率升高,HK-Ⅱ、LDH、β-catenin、cmyc水平均降低,差异均有统计学意义(P﹤0.05)。si RNA control组细胞凋亡率、HK-Ⅱ、LDH、β-catenin、c-myc水平与对照组比较,差异无统计学意义(P﹥0.05)。结论宫颈癌细胞中下调NOB1表达后,细胞凋亡增加,细胞糖酵解水平下降,这可能由于NOB1对Wnt/β-catenin信号通路具有调控作用。Objective To investigate the effect of NOB1 on apoptosis and glycolysis in cervical cancer cells. Method Cervical cancer cells He La transfected with NOB1 siRNA and siRNA control were named as NOB1 siRNA group and siRNA control group, the untransfected cells were used as controls. Transfection effects were detected by RT-PCR and Western blot, cell apoptosis was detected by flow cytometry, HK-II and LDH activity were determined by assay kit, the expression of β-catenin and c-myc protein was detected by Western blot. Result There was no significant difference in the level of NOB1 mRNA and protein in siRNA control group compared with the control group（P〉0.05）; while the level of NOB1 mRNA and protein in NOB1 siRNA group was significantly lower than that of the control group, with statistically significant difference observed（P〈0.05）. The apoptosis rate of NOB1 siRNA cells increased significantly, and the level of HK-II, LDH, β-catenin and c-myc decreased significantly, showing significant difference as compared with the control group（P〈0.05）. The apoptosis rate, level of HK-II, LDH, β-catenin, and c-myc did not significantly differ between siRNA control group and control group（P〉0.05）. Conclusion Downregulation of NOB1 expression in cervical cancer cells induces increased cell apoptosis and reduced glycolysis, which may be due to the regulation of Wnt signaling pathway by NOB1.

关 键 词：NOB1 宫颈癌 糖酵解 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]