CHX等离子体杀灭牙龈卟啉单胞菌的实验研究  

The antimicrobial activity of plasma jet with helium flowing through 2% chlorhexidine against Porphyromonas gingivalis

作　　者：周鑫才[1] 梁徳凤 陈伟建 张文浩 曹颖光[2] 卢新培[3] ZHOU Xin- cai;LIANG De- feng;CHEN Wei- fian;ZHANG Wen- hao;CAO Ying-guang;LU Xin- pei(Department of Stomatology, Baoan Maternal and Child Health Hospital, College of Medicine, Jinan University, Gnangdong Shenzhen 518133, China;Department of Stomatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology ,Hubei Wuhan 430030, China;College of Electrical and Electronic Engineering,Huazhong University of Scienee and Technology, Hubei Wuhan 430074, China.)

机构地区：[1]暨南大学医学院附属宝安妇幼保健院口腔科,广东深圳518133 [2]华中科技大学同济医学院附属同济医院口腔医学中心,湖北武汉430030 [3]华中科技大学电气和电子工程学院,湖北武汉430074

出　　处：《临床口腔医学杂志》2018年第6期341-344,共4页Journal of Clinical Stomatology

基　　金：深圳市科技创新委员会基金支持(JCYJ20170307161031266);中国疾病预防控制中心妇幼保健中心合生元母婴营养与健康研究项目资助(2017FYH004)

摘　　要：目的:了解氯已定(chlorhexidin,CHX)等离子体杀灭牙龈卟啉单胞菌的效果。方法:将牙龈卟啉单胞菌混悬液接种于30个无菌盖玻片上,培养10 d,按照氦气是否通过2%CHX、是否产生等离子体以及作用时间随机分为1个对照组和4个实验组,每组6个,分别作用后,平板计数计算活菌的量。结果:CHX等离子体和等离子体都有较好的杀灭牙龈卟啉单胞菌的效果,与对照组相比,差异均有统计学意义(P<0.05)。CHX等离子体作用4 min组和2 min组的细菌数量分别比对照组下降4.795和2.827个对数单位CFU;等离子体作用4 min组和2 min组的细菌数量分别比对照组下降3.795和1.291个对数单位CFU。CHX等离子体作用4 min组与CHX等离子体作用2min组、等离子体作用4 min组和2 min组相比,细菌数量下降更多,差异均有统计学意义(P<0.05)。结论:CHX等离子体能有效的杀灭牙龈卟啉单胞菌,可考虑将用于种植体周围炎的治疗。Objective： To investigate the antimicrobial activity of plasma jet with helium（ He） flowing through 2%chlorhexidine（ CHX） against Porphyromonas gingivalis. Methods： Porphyromonas gingivalis suspensions were inoculated into30 sterile cover slips for incubation to establish the bacterial biofilms. The infected specimens were randomly divided into one control group treated without plasma and four experimental groups,that He gas flowing for 4 min with the plasma off（ Group1）,plasma jet sterilization without He gas flowing through 2% CHX for 2 min（ Group 2）,or 4 min（ Group 3）,and plasma jet sterilization with He gas flowing through 2% CHX for 2 min（ Group 4）,or 4 min（ Group 5）. The number of surviving bacteria in each specimen was determined by counting the colony forming units（ CFU）/m L on anaerobic petri dish. Results： The results indicated that statistically significant reduction in CFU/m L（ P〈0. 05） existed for four treatment groups compared to the control group. The reductions in CFU/m L were observed for Group 5（ 4. 795 log unit reduction） and Group 4（ 2. 827 log unit reduction）,which were treated by plasma jet sterilization with He flowing through 2% chlorhexidine for 4 or 2 min,respectively. In addition,the reduction in Group 5 was significantly greater compared with that in Group 4 or in the groups treated by plasma jet sterilization without He flowing through 2% chlorhexidine for 2 or 4 min（ P〈0. 05）. Conclusion： Plasma jet with He flowing through 2% chlorhexidine can effectively sterilized porphyromonas gingivalis and should be considered as an alternative method for peri-implantitis.

关 键 词：抗菌效果 等离子体射流 氯己定 牙龈卟啉单胞菌 

分 类 号：R780.2[医药卫生—口腔医学]