酸胁迫下琥珀酸放线杆菌的生理及转录应答  

Physiological and transcriptional responses of Actinobacillus succinogenes to acid stress

作　　者：张群 陈鹏程[1,2] 郑璞 Qun Zhang;Pengcheng Chen;Pu Zheng(Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu Province, China;School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu Province, China)

机构地区：[1]江南大学工业生物技术教育部重点实验室,江苏无锡214122 [2]江南大学生物工程学院,江苏无锡214122

出　　处：《微生物学报》2018年第7期1255-1265,共11页Acta Microbiologica Sinica

基　　金：国家自然科学基金(21604032);"十二五"农村领域国家科技计划课题(2015BAD15B04);中央高校基本科研业务费专项资金(JUSRP116031);江南大学工业生物技术教育部重点实验室开放课题(KLIB-KF201501)~~

摘　　要：【目的】通过琥珀酸放线杆菌Actinobacillus succinogenes CGMCC1593对酸胁迫的生理应答和转录组学分析,探究琥珀酸放线杆菌酸胁迫的机制。【方法】测定不同pH对细胞生长、H+-ATPase、细胞内pH的影响;测定酸胁迫前后细胞膜和谷氨酸脱氢酶的变化、谷氨酸对琥珀酸放线杆菌生长的影响;通过RNA-seq测序分析酸胁迫条件下的差异表达基因。【结果】随pH值的降低,细胞生长受抑制,H+-ATPase的活性下降。pH 4.7酸胁迫后,细胞膜受到严重损伤,谷氨酸对酸胁迫后的细胞有保护作用,GDH酶活响应酸胁迫后略有增加。酸胁迫后,39个基因差异表达较为显著,其中49%基因属于应激蛋白、转运蛋白,小部分基因与代谢相关。【结论】本文探究了琥珀酸放线杆菌酸胁迫下的生理及转录应答,研究结果可为寻找增强琥珀酸放线杆菌耐酸性策略提供参考。[Objective] We explored by physiological and transcriptional responses of Actinobacillus succinogenesCGMCC1593 to acid stress.[Methods] We studied the effects of different initial p H on cell growth,activity of H＋-ATPase and intracellular p H before and after acid stress as well as the protection mechanism of glutamate on CGMCC1593.The transcriptomics technique was used to explore the differentially expressed genes under acid stress.[Results] Cell growth was inhibited and the activity of H＋-ATPase decreased with the decrease of initial p H.After acid stress at p H 4.7,the cell membrane was severely damaged.Glutamate had a protective effect on the cells under acid stress.In total 39 genes were differently expressed altogether under acid stress,of which 49% were stress and transport proteins,and a small part was related to metabolism.[Conclusion] Understanding the physiological and transcriptional responses of Actinobacillus succinogenes to acid stress provides references for improving acid resistance of Actinobacillus succinogenes.

关 键 词：琥珀酸放线杆菌 酸胁迫 细胞膜 细胞内pH H^+-ATPASE GDH RNA-SEQ 

分 类 号：Q935[生物学—微生物学]