高脂软饲料增加肥胖复合牙周炎小鼠模型丝线松脱率的研究  

High-fat soft diet increases silk ligature detachment rate in combined obesity and periodontitis mouse models

作　　者：余挺[1] 赵莉[2] 章锦才 轩东英 YU Ting;ZHAO Li;ZHANG Jincai;XUAN Dongying(Department of Periodontology, Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China)

机构地区：[1]广州医科大学附属口腔医院牙周科、广州口腔疾病研究所,口腔医学重点实验室,广东广州510140 [2]中山大学光华口腔医学院、附属口腔医院口腔修复科、广东省口腔医学重点实验室,广东广州510055 [3]中国科学院大学存济医学院,北京100049 [4]中国科学院大学存济医学院、杭州口腔医院牙周科,浙江杭州310006

出　　处：《口腔医学》2018年第6期491-494,共4页Stomatology

基　　金：国家自然科学基金(81700985;81371151;81470750)

摘　　要：目的探索高脂软饲料对肥胖复合结扎诱导牙周炎模型的丝线稳定性的影响。方法将134只小鼠分为高脂(HFD,n=77)和低脂饲料(LFD,n=57)组,分别诱导肥胖和对照组。诱导8、16、30周时,一次性丝线结扎5 d或10 d诱导牙周炎。分析饲料类型、饲料诱导期、牙周结扎期对丝线松脱的效应。结果丝线总体松脱率为5.97%,10 d(8.04%)比5 d(2.50%)高2倍(P<0.01),HFD(9.09%)比LFD(1.75%)高4倍(P<0.05)。饲料诱导期对松脱率无显著影响。结论建立饮食诱导型肥胖与丝线结扎诱导牙周炎的复合模型时,高脂软饲料可明显增加丝线松脱率。为调和保护肥胖状态与结扎期延长致丝线松脱率增加的矛盾,结扎10 d时结束诱导或更换丝线是合适的时间节点。Objective To explore impacts of high-fat diet on stability of silk ligature in combined obesity and ligation-induced periodontitis mouse models. Methods One hundred and thirty-four mice were divided into high-fat（ HFD,n = 77） and low-fat diet（ LFD,n= 57） groups to induce obesity and normal-weight control,respectively. After 8,16 or 30 weeks on diet,all mice were induced periodontitis with silk by one-time ligation for 5 or 10 days. Effects of diet type,duration on diet and ligation duration on ligature detachment were statistically analyzed. Results The total detachment rate of ligature was 5.97%. The 10-day group had 2 times higher（ 8.04% vs. 2.50%,P〈0.01） and the HFD group had 4 times higher（ 9.09% vs. 1.75%,P〈0.05） detachment rates compared with those of 5-day and LFD groups,respectively. Duration on diet had no significant effect on detachment rate. Conclusion When establishing combined models of diet-induced obesity and ligation-induced periodontitis,high-fat soft diet significantly increases detachment of silk ligature. To reconcile the contradiction between protecting obese condition and increased ligature detachment due to overtime ligation,10 days＇ ligation is an appropriate threshold to stop induction or to change silk ligature.

关 键 词：肥胖 牙周炎 动物模型 丝线 结扎 脱落 饲料 

分 类 号：R781.4[医药卫生—口腔医学]