牙龈卟啉单胞菌刺激树突状细胞激活炎症反应  

Porphyromonas gingivalis stimulates dendritic cells to activate inflammatory responses

作　　者：宋亮 徐斌 刘阳 刘柳慧 郑义彩 肖国岫 施晓军 SONG Liaag;XU Bin;LIU Yang;LIU Liuhui;ZHENG Yicai;XIAO Guoxiu;SHI Xiaojun(Department of Stomatology, the Fifth People＇s Hospital of Shanghai, Fudan University, Shanghai 200240, Chin)

机构地区：[1]复旦大学附属上海市第五人民医院口腔科,上海200240 [2]复旦大学生物医学研究院,上海200032

出　　处：《口腔医学》2018年第6期503-507,共5页Stomatology

基　　金：上海市卫生局课题(201740230);上海市闵行区科委课题(2014MHZ050);复旦大学附属上海市第五人民医院菁英人才培养项目(2017WYRCJY02)

摘　　要：目的分析树突状细胞在牙龈卟啉单胞菌感染后的差异表达基因,探讨牙周炎的致病机制。方法利用高通量测序技术分析体外诱导分化的树突状细胞在牙龈卟啉单胞菌感染情况下的差异基因表达、基因集及相关功能。结果本实验总计发现7 305个至少有两倍变化的差异表达基因。基因本体结果显示牙龈卟啉单胞菌感染后树突状细胞的生物过程、细胞成份、分子功能均发生较大变化。基因集富集分析结果显示肿瘤坏死因子基因集、细胞因子及炎症反应基因集、白介素1及转录因子NF-k B家族基因集、干扰素信号基因集、B细胞受体信号基因集、T细胞受体信号基因集富集明显。结论树突状细胞在牙龈卟啉单胞菌感染后会导致基因水平的变化,引起机体免疫反应及炎症反应的发生,趋化因子配体(Chemokine(C-X-C motif)ligand,CXCL)及细胞因子信号抑制物(Suppressor of cytokine signaling,SOCS)的水平及作用可作为日后研究的方向。Objective To analyze differentially expressed genes of dendritic cells infected with Porhyromonas gingivalis（ P.gingivalis）,to explore the pathogenesis of periodontitis. Methods High-throughput sequencing was used to analyze differentially expressed genes,gene pools and related functions of in vitro induced dendritic cells under P. gingivalis infection. Results A total of 7305 differentially expressed genes with at least two-fold changes were found in this experiment. The results of gene ontology showed that the biological processes,cell components,and molecular functions of dendritic cells after P. gingivalis infection greatly changed. Gene enrichment analysis showed that the tumor necrosis factor gene set,cytokine and inflammatory response gene set,interleukin 1 and NF-k B family of gene transcription factor,interferon signal gene set,B cell receptor signal gene set,T cell receptor signal gene set were enriched significantly. Conclusions Dendritic cells can cause genetic changes after P. gingivalis infection,causing the immune response and inflammatory reaction. The levels and effects of CXCL and SOCS can be the direction of future research.

关 键 词：牙龈卟啉单胞菌 树突状细胞 牙周炎 免疫反应 

分 类 号：R780.2[医药卫生—口腔医学]