骨髓来源的抑制性细胞及亚群在炎症性肠病中的变化及意义  

Clinical significance of changes of myeloid-derived suppressor cells and their subtypes in inflammatory bowel disease

作　　者：贺宜 欧阳春晖[1] 卢放根[1] 吴小平[1] 张洁[1] He Yi;Ouyang Chunhui;Lu Fanggen;Wu Xiaoping;Zhang Jie(Department of Gastroenterology, 2nd Xiangya Hospital of Central South University, Changsha 410011, China)

机构地区：[1]中南大学湘雅二医院消化内科,长沙410011

出　　处：《中华消化杂志》2018年第5期329-333,共5页Chinese Journal of Digestion

基　　金：国家自然科学基金(81470801)

摘　　要：目的探讨IBD患者外周血中骨髓来源的抑制性细胞（MDSC）及亚群的变化和临床意义。方法选取2016年4月至2017年4月中南大学湘雅二医院的99例IBD住院患者为观察组1，其中CD患者84例（活动期70例，缓解期14例），UC患者15例，同期选择经询问病史及体格检查正常的32名健康人群为健康对照组1，检测观察组1CD、UC患者和健康对照组1外周血MDSC及亚群所占比例。从观察组1中选取62例IBD患者（47例CD患者，15例UC患者）为观察组2，从健康对照组1中选取21名健康人群为健康对照组2，检测血清TNF-α和IL-8水平。统计学方法采用卡方检验、t检验和单因素方差分析，相关性采用Pearson相关性分析。结果观察组1中CD和UC患者MDSC在外周血单个核细胞中的比例均高于健康对照组1[（6.30±3.97）%和（7.50±3.12）%比（3.94±2.25）%]，差异均有统计学意义（t＝－3.22、－3.21，P均〈0.01）。CD患者粒细胞型MDSC在外周血单个核细胞中的比例高于UC患者和健康对照组1[（65.69±20.45）%比（50.93±13.56）%和（51.50±11.61）%]，差异均有统计学意义（t＝2.93、3.79，P均〈0.01）。UC患者单核细胞型MDSC在外周血单个核细胞中的比例高于CD患者和健康对照组1[（28.41±18.33）%比（18.38±17.43）%和（28.17±10.22）%]，差异均有统计学意义（t＝2.01、2.53，P均〈0.05）。CD活动期患者粒细胞型MDSC在外周血单个核细胞中的比例高于缓解期患者，CD活动期患者单核细胞型MDSC在外周血单个核细胞中的比例均低于缓解期患者[分别为（67.36±2.27）%比（46.49±6.32）%，（17.19±2.02）%比（34.33±6.12）%]，差异均有统计学意义（t＝3.60、3.26，P均〈0.01）。观察组2中CD患者中TNF-α水平高于UC患者和健康对照组2[（7.83±6.54）ng/L比（4.77±2.12）ng/L和（4.40±2.05）ng/L]，差异均有统计学意义（t＝2.01、2.53，P均〈0.05）；观察组2中UC患者IL-8水平高ObjectiveTo explore the changes and their clinical significance of peripheral blood myeloid-derived suppressor cells （MDSC） and their subtypes in patients with inflammatory bowel disease （IBD）.MethodsFrom April 2016 to April 2017, 99 hospitalized IBD patients in 2nd Xiangya Hospital of Central South University were enrolled as observation group one, which included 84 Crohn′s disease （CD） （70 in active phase and 14 in remission phase） and 15 patients with ulcerative colitis（UC）. At the same period, 32 healthy controls were enrolled as healthy control group one. The proportion of peripheral blood MDSC and subtypes of CD patients, UC patients and healthy controls were examined. Observation group two including 62 IBD patients （47 CD and 15 UC） were selected from observation group one and 21 healthy individuals were selected from healthy control group one as healthy control. The serum levels of tumor necrosis factor α （TNF-α） and interleukin 8 （IL-8） were detected. Chi square test, t test and one-way analysis of variance were performed for statistical analysis. Pearson correlation was performed for correlation analysis.ResultsThe proportion of MDSC in peripheral blood mononuclear cells of CD and UC patients of observation group one were both higher than that of healthy control group one （（6.30±3.97）% and （7.50±3.12）% vs. （3.94±2.25）%, respectively）, and the differences were statistically significant （t=-3.22 and -3.21, both P〈0.01）. The proportion of granulocytic MDSC in peripheral blood mononuclear cells of CD patients was higher than that of UC patients and healthy control group one （（65.69±20.45）% vs. （50.93±13.56）% and （51.50±11.61）%, respectively）, and the differences were statistically significant （t=2.93 and 3.79, both P〈0.01）. The proportion of monocytic MDSC in peripheral blood mononuclear cells of UC patients was higher than that of CD patients and healthy control group one （（28.41±18.33）% vs. （18.38±17.43）% and

关 键 词：炎性肠疾病 肿瘤坏死因子Α 白细胞介素8 骨髓来源的抑制性细胞及亚群 

分 类 号：R574[医药卫生—消化系统]