规范标本采集及送检流程对提高甲型流感病毒快速筛查阳性率的探讨与分析  

Discussion and analysis on the effection of standardcollection and shipment ofsampleson increasing positive rate of influenza A virus

作　　者：郭阳[1] 苑晓冬[1] 佟丽娟 孙玉玲[1] 王海英[1] 乌日乐 栾颖[1] GUO Yang;YUAN Xiao-dong;TONG Li-juan;SUN Yu-ling;WANG Hai-ying;WU Ri-le;LUAN Ying.(Beijing Shijitan Hospital, Capital Medical University ,Beijing 100038,China)

机构地区：[1]首都医科大学附属北京世纪坛医院感染科,北京100038

出　　处：《中国急救复苏与灾害医学杂志》2018年第6期547-548,共2页China Journal of Emergency Resuscitation and Disaster Medicine

摘　　要：目的探讨医务人员通过正确规范执行咽拭子标本采集及合理送检流程能否提高咽拭子快速检测甲型流感病毒抗原的阳性率。方法对比2016年1月1日-2017年3月1日首都医科大学附属北京世纪坛医院由发热门诊和其他科室分别采集的咽拭子样本，发热门诊样本直接送检，其他科室样本由外勤人员负责运送，所有标本均送至发热门诊检验室并运用“甲型流感病毒抗原检测试剂盒（DOT—ELISA法）”即刻检测。结果发热门诊共采集口咽拭子标本1539例，分离流感病毒阳性者为152例，阳性率9．88％，其他科室共采集口咽拭子标本928份，分离流感病毒阳性者68例，阳性率7．33％。发热门诊甲型流感病毒抗原检测阳性率高于其他科室，差异有统计学意义（P〈0．05）。结论正确规范的咽拭子标本采集并及时严格送检样品，可提高甲型流感检测的阳性率。Objective To discuss theeffection of standardcollection and shipmentofsampleson increasing positive rate of influenza A virus. Methods Nasopharyngeal swab specimens from influenza patients were collected by doctors. The specimens from fever outpatient were transmitted tolaboratoryby doctors, and the specimens from other department were transmitted to laboratory by outworkers. All samples were tested using influenza A virus antigen test kit （DOT-ELISA method）.Results A total of 1539 nasopharyngeal swab specimens were collected at fever outpatient from January 1, 2016 to March 1, 2017, and 152 influenzaA-Ag positive patients were found, the positive rate was 9.88%.A total of 928 nasopharyngeal swab specimens were collected at other department, 68 influenzaA-Ag positive patients were found, the positive rate was 7.33%. The positive rate of fever outpatient was higher than other department, and the difference was statistically significant （P 〈0.05）.Conclusion Correct and normativeapproachofspecimencollection and sending samples in time can increasing the rate of detection of influenza A virus.

关 键 词：鼻咽拭子 甲型流感病毒 标本采集 

分 类 号：R739.63[医药卫生—肿瘤]