脑脊液细胞学检查联合细胞免疫化学染色在脑膜癌病诊断中的价值  

The value of cerebrospinal fluid cytology and cell immunochemical staining in diagnosing meningeal carcinomatosis

作　　者：姜宇[1] 周仁华[1] 王娟[2] 沈骏[1] 鞠强国 袁颖[1] Jiang Yu;Zhou Renhua;Wang Juan;Shen Jun;Ju Qiangguo;Yuan Ying(Department of Neurology, the Fourth People＇s Hospital of Taizhou, Jiangsu Taizhou 225300, Chin)

机构地区：[1]江苏省泰州市第四人民医院神经内科,225300 [2]南京医科大学附属南京脑科医院病理科

出　　处：《中国医师进修杂志》2018年第4期308-310,共3页Chinese Journal of Postgraduates of Medicine

摘　　要：目的 探讨脑脊液细胞学检查联合细胞免疫化学染色方法在临床追踪脑膜癌病（MC）中的诊断价值.方法 患者脑脊液采用StatSpin Cytofuge 12离心机离心沉淀,MGG染色和SP法细胞免疫化学染色,对确诊为脑膜癌病的16例患者临床资料进行回顾性分析.结果 16例患者中11例通过脑脊液细胞学检查联合细胞免疫化学染色确诊MC先于影像学发现脑膜病变;脑脊液细胞学检查光镜下观察癌细胞异型性明显,细胞免疫学检查清晰分辨MC的组织来源分类.结论 脑脊液细胞学检查联合细胞免疫化学染色方法是追踪颅内转移性恶性肿瘤的重要临床手段之一.Objective To explore the diagnostic value of cytologic examination of cerebrospinal fluid （CSF） combined with cell immunochemical staining examination in the patients with meningeal carcinomatosis in the clinical track.Methods CSF was centrifuged and precipitated using the StatSpin Cytofuge 12 centrifuge. It was dyed by the May-Grunwald-Giemsa Staining （MGG） method and cell immunochemical staining （S-P method）. Clinical data of 16 cases with the diagnosis of meningeal carcinomatosis were retrospectively analyzed. Results Sixteen patients diagnosed meningeal carcinomatosis prior to imaging findings in 11 of 16 patients were diagnosed meningeal carcinomatosis through the cerebrospinal fluid cytology combined with cell immunochemical staining prior to imaging findings. CSF cytology observed the cancer cell pleomorphism. Cell immunochemical staining examination clearly distinguished classification organic source of meningeal carcinomatosis. Conclusions CSF cytology combined with cell immunochemical staining examination is one of the important means in tracking intracranial metastatic tumor in clinic.

关 键 词：脑膜癌病 硬膜下积液 细胞学技术 细胞免疫化学染色 

分 类 号：R739.45[医药卫生—肿瘤]