大恒优质肉鸡慢羽性状的分子鉴定方法研究  

Study on Molecular Identification Techniques for Slow-feathering Character of Daheng Meat-quality Broilers

作　　者：邱莫寒[1] 马恩跃 杜华锐[1] 杨朝武[1] 朱师良 罗彬 张增荣[1] 王彦 许恒勇 蒋小松[1] QIU Mohan;MA Enyue;DU Huarui;YANG Chaowu;ZHU Shiniang;LUO Bin;ZHANG Zengrong;WANG Yan;XU Hengyong;JIANG Xiaosong(Key Laboratory of Animal Breeding and Genetics of Sichuan Province, Sichuan Academy of Agricultural Sciences, Chengdu, Sichuan 610066;Key Laboratory of Farm Animal Genetic Resources Exploration and Innovation of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan 611130)

机构地区：[1]四川省畜牧科学研究院,四川省动物遗传育种重点实验室,四川成都610066 [2]四川农业大学畜禽遗传资源发掘与创新利用四川省重点实验室,四川成都611130

出　　处：《中国家禽》2018年第11期5-8,共4页China Poultry

摘　　要：为了建立一种快速准确鉴定大恒优质肉鸡慢羽系公鸡纯合子的分子检测方法,以加快纯合慢羽系及其配套系的育种速度,利用鸡性连锁羽速基因K/k+中慢羽基因的断点连接序列以及快慢羽基因的SNP,选择200只(表型鉴定为慢羽)50日龄的大恒优质肉鸡慢羽系公鸡进行羽速基因的分子检测,检测结果与雏鸡羽速表型判定结果一致率达100%。采用限制性内切酶TaqⅠ法进一步检测发现,慢羽纯合子公鸡(197只)2条带,杂合子公鸡(3只)3条带。综上,可以利用该方法鉴定大恒优质肉鸡慢羽系公鸡基因型是否为纯合的慢羽个体。The study was aimed to establish a rapid and accurate molecular identification techniques for homozygote individual of slow-feathering Daheng meat-quality male broilers to speed up the breeding of slow-feathering pure and crossbreed using the relation of slow-feathering gene in linkage with feathering gene K/k＋ and single nucleotide polymorphisms. The feathering phenotypes were detected in Daheng meat-quality broilers for 200 slow-feathering phenotypic individuals at 50 days of age. In order to verify homozygote of genotype of slow-feathering cock, the amplified products were digested using the restriction enzyme Taq I. The results showed that homozygous of slow-feathering Daheng male chicken had two bands （197）and the individuals with three bands were heterozygous （3）. Therefore, this method could be used for identifying homozygous genotype of Daheng male chicken.

关 键 词：大恒优质肉鸡 K等位基因 慢羽 纯合子 分子鉴定 

分 类 号：S831.2[农业科学—畜牧学]