响应面法优化菊花黄酮超声-微波辅助提取工艺及其抗氧化活性  

Optimization of ultrasonic-microwave assisted extraction of flavonoids fromChrysanthemums by response surface methodology and its antioxidant activity

作　　者：皇甫阳鑫 高子怡 展俊岭 任海仙[1] 赵二劳[1] HUANGFU Yang-xin;GAO Zi-yi;ZHAN Jun-ling;REN Hai-xian;ZHAO Er-lao(Department of Chemistry, Xinzhou Teachers University, Xinzhou 034000, Chin)

机构地区：[1]忻州师范学院化学系

出　　处：《分子科学学报》2018年第3期251-257,共7页Journal of Molecular Science

基　　金：国家级大学生创新创业训练计划项目(201610124001);山西省高等学校大学生创新创业训练计划项目(2018507);忻州师范学院化学化工创新基地项目(2018)

摘　　要：以黄酮提取率为指标,采用单因素实验结合响应面法优化菊花黄酮的超声-微波辅助提取工艺,并研究了所提菊花黄酮的抗氧化活性.结果表明,菊花黄酮超声-微波辅助提取最佳工艺条件为：乙醇体积分数为76%,料液比为1∶52（g/mL）,超声功率为200 W,超声时间为43min.在此工艺条件下,菊花黄酮提取率为10.623%.菊花黄酮对DPPH·和·OH清除能力明显强于BHT,表明菊花黄酮具有较强的抗氧化活性.The chrysanthemums flavonoids ultrasonic-microwave assisted extraction process was optimized by single-factor and response surface methodology using the extraction rate of flavonoids as indexes,and studied its antioxidant activity.The results showed that the optimal extraction conditions of flavonoids by the ultrasonic-microwave assisted method were determined as follows：ethanol volume fraction was 76%,the ratio of solid to solvent was 1∶52（g/mL）,microwave power was 200 Wand retraction time was 43 min.Under the optimal conditions,the extraction rate of flavonoids was10.623%,and the scavenging ability to DPPH·and·OH was significantly stronger than that of BHT solution.These demonstrated that chrysanthemums flavonoids possessed strong antioxidant activity.

关 键 词：菊花 黄酮 响应面法 超声-微波辅助 抗氧化活性 

分 类 号：O658[理学—分析化学]