7份贵州禾香味鉴定及OsBADH2基因CDS差异分析  

The CDS Variance Analysis of OsBADH2 Gene and Aroma Appraisal of 7 Guizhou HE(Oryza sativa)

作　　者：王丽丽[1] 彭强 李佳丽 张大双 吴健强 姜雪 朱速松[1] Wang Lili;Peng Qiang;Li Jiali;Zhang Dashuang;Wu Jianqiang;Jiang Xue;Zhu Susong(Guizhou Rice Research Institute, Guizhou Academy of Agricultural Science, Guiyang, 55000)

机构地区：[1]贵州省农业科学院水稻研究所,贵阳550006

出　　处：《分子植物育种》2018年第13期4138-4142,共5页Molecular Plant Breeding

基　　金："十二五"农村领域国家科技计划课题(2014AA10A604-11);贵州省联合基金项目(黔科合LH字(2015)7074号);贵州省重大专项黔科(合重大专项字(2013)6023);贵州省科技支撑计划项目(黔科合支撑(2018)2298);省科研机构服务企业行动计划(黔科合服企(2014)4005);贵州省科学技术基金(黔科合J字(2015)2098号)共同资助

摘　　要：水稻OsBADH2基因功能丧失是稻米产生香味的内因。本研究对7份贵州禾和2份对照品种的香味性状进行物化鉴定,发现榕禾与2个对照品种为香稻,其余6份贵州禾都为非香稻。为解析贵州禾产生香味与OsBADH2基因的内在关系,通过TA克隆、测序分析和序列拼接,本研究获得9份材料OsBADH2基因CDS的序列信息,采用Megalign软件对其进行序列比对分析。结果表明:4份贵州禾(白香禾2号、半坡禾、黎平禾1号和黎平禾2号)的OsBADH2基因CDS无突变;榕禾和2个对照品种的CDS差异都为第7外显子的8 bp缺失和3 SNP;还发现2种新的CDS差异(白香禾1号为1 bp缺失和1 SNP,便禾为2 bp缺失和2 SNP)。这些结果为贵州禾香味品种保护及育种改良提供理论基础。Dysfunction of OsBADH2 gene in rice is the internal cause of rice to have fragrance. This study applied physicochemical methods to identify the fragrance trait of 7 Guizhou HE（Oryza sativa） and 2 control varieties. It was found that Rong He and 2 control varieties were fragrant rice, and the other 6 varieties were all non-fragrant rice.To analysis the internal relationship between fragrant production in Guizhou HE and OsBADH2 gene, the study obtained the CDS sequences of OsBADH2 gene in 9 tested materials, and conducted alignment analysis of these sequences by Megalign software through TA-cloning, sequencing analysis and sequence assembly. The results showed that the OsBADH2 gene CDS of 4 Guizhou HE（Baixianghe 2, Banpo He, Liping He 1 and Liping He 2） were normal. The CDS differences of Rong He and 2 control varieties were both 8 bp deletion and 3 SNP in exon 7, and2 new CDA differences were found which Baixianghe 1 was 1 bp deletion and 1 SNP, and Bian He was 2 bp deletion and 2 SNP. These results might provide the theoretical basis for varieties protection and breeding improvement of Guizhou HE（Oryza sativa）.

关 键 词：水稻 TA克隆 序列拼接 香味 

分 类 号：S511[农业科学—作物学]